BillionToOne, Inc. 10-Q

Item 1. Financial Statements (Unaudited) BillionToOne, Inc. Balance Sheets (in thousands, except share amounts, unaudited) June 30, 2026 December 31, 2025 Assets Current assets: Cash and cash equivalents $ 548,628 $ 495,975 Accounts receivable 74,894 41,617 Inventories 21,135 17,545 Prepaid expenses and other current assets 6,719 5,421 Total current assets 651,376 560,558 Property and equipment, net 24,932 20,361 Operating lease right-of-use assets, net 47,829 46,742 Other non-current assets 5,559 4,993 Total assets $ 729,696 $ 632,654 Liabilities and stockholders’ equity Current liabilities: Accounts payable $ 12,205 $ 7,184 Accrued expenses and other current liabilities 12,603 7,247 Accrued commissions 3,833 3,912 Accrued compensation and employee benefits 18,000 12,551 Common stock warrant liability - 9,282 Deferred revenue, current 2,328 2,188 Operating lease liabilities, current 6,343 5,079 Financing lease liabilities, current 413 519 Total current liabilities 55,725 47,962 Operating lease liabilities, non-current 45,981 45,723 Financing lease liabilities, non-current 153 348 Deferred revenue, non-current - 1,290 Long-term debt 91,031 57,226 Total liabilities 192,890 152,549 Commitments and contingencies (Note 5) Stockholders’ equity: Class A common stock, $ 0.00001 par value, 800,000,000 shares authorized as of June 30, 2026 and December 31, 2025; 42,283,900 and 41,252,105 shares issued and outstanding as of June 30, 2026 and December 31, 2025, respectively - - Class B common stock, $ 0.00001 par value; 10,000,000 shares authorized as of June 30, 2026 and December 31, 2025; 4,542,650 and 4,552,650 shares issued and outstanding as of June 30, 2026 and December 31, 2025, respectively - - Additional paid-in capital 787,321 756,644 Accumulated other comprehensive loss ( 1,792 ) ( 1,792 ) Accumulated deficit ( 248,723 ) ( 274,747 ) Total stockholders’ equity 536,806 480,105 Total liabilities and stockholders’ equity $ 729,696 $ 632,654 The accompanying notes are an integral part of these interim financial statements. 4 Table of Contents BillionToOne, Inc. Statements of Operations and Comprehensive Income (Loss) (in thousands, except per share amounts, unaudited) Three Months Ended June 30, Six Months Ended June 30, 2026 2025 2026 2025 Revenue $ 109,448 $ 66,573 $ 217,836 $ 125,536 Cost of revenue 32,341 23,107 61,633 44,098 Gross profit 77,107 43,466 156,203 81,438 Operating expenses: Research and development 17,315 11,751 32,007 22,181 Selling, general and administrative 54,289 33,342 100,859 63,199 Total operating expenses 71,604 45,093 132,866 85,380 Income (loss) from operations 5,503 ( 1,627 ) 23,337 ( 3,942 ) Other income (expense): Interest income 4,694 1,451 9,339 2,957 Interest expense ( 8 ) ( 32 ) ( 18 ) ( 72 ) Change in fair value of term loan ( 2,968 ) ( 10 ) ( 7,229 ) ( 3,102 ) Other income (expense), net 297 ( 14 ) 598 39 Total other income (expense) 2,015 1,395 2,690 ( 178 ) Income (loss) before provision for income taxes 7,518 ( 232 ) 26,027 ( 4,120 ) Provision for income taxes ( 536 ) 14 3 114 Net income (loss) and comprehensive income (loss) $ 8,054 $ ( 246 ) $ 26,024 $ ( 4,234 ) Net income (loss) per share, basic and diluted: Net income (loss) per share, basic $ 0.17 $ ( 0.02 ) $ 0.56 $ ( 0.41 ) Net income (loss) per share, diluted $ 0.15 $ ( 0.02 ) $ 0.48 $ ( 0.41 ) Weighted-average shares used in calculating net income (loss) per share, basic and diluted: Weighted-average shares used in calculating net income (loss) per share, basic 46,403,350 10,385,000 46,169,335 10,349,146 Weighted-average shares used in calculating net income (loss) per share, diluted 54,455,523 10,385,000 53,743,241 10,349,146 The accompanying notes are an integral part of these interim financial statements. 5 Table of Contents BillionToOne, Inc. Statements of Redeemable Convertible Preferred Stock and Stockholders’ Equity (Deficit) (in thousands, except share data, unaudited) Three Months Ended June 30, 2026 Redeemable Convertible Preferred Stock Common Stock Additional Paid-In Capital Accumulated Other Comprehensive Loss Accumulated Deficit Total Stockholders' Equity Shares Amount Shares Amount Balance at March 31, 2026 - $ - 45,980,892 $ - $ 764,189 $ ( 1,792 ) $ ( 256,777 ) $ 505,620 Issuance of Class A common stock upon exercise of stock options - - 732,542 - 6,430 - - 6,430 Stock-based compensation - - - - 7,482 - - 7,482 Exercise of Class A common stock warrants - - 113,116 - 9,220 - - 9,220 Net income - - - - - - 8,054 8,054 Balance at June 30, 2026 - $ - 46,826,550 $ - $ 787,321 $ ( 1,792 ) $ ( 248,723 ) $ 536,806 Three Months Ended June 30, 2025 Redeemable Convertible Preferred Stock Common Stock Additional Paid-In Capital Accumulated Other Comprehensive Loss Accumulated Deficit Total Stockholders' Deficit Shares Amount Shares Amount Balance at March 31, 2025 29,084,235 $ 419,409 11,056,337 $ - $ 33,284 $ - $ ( 286,189 ) $ ( 252,905 ) Issuance of common stock upon exercise of stock options - - 28,707 - 166 - - 166 Stock-based compensation - - - - 2,732 - - 2,732 Net loss - - - - - - ( 246 ) ( 246 ) Balance at June 30, 2025 29,084,235 $ 419,409 11,085,044 $ - $ 36,182 $ - $ ( 286,435 ) $ ( 250,253 ) Six Months Ended June 30, 2026 Redeemable Convertible Preferred Stock Common Stock Additional Paid-In Capital Accumulated Other Comprehensive Loss Accumulated Deficit Total Stockholders' Equity Shares Amount Shares Amount Balance at December 31, 2025 - $ - 45,804,755 $ - $ 756,644 $ ( 1,792 ) $ ( 274,747 ) $ 480,105 Issuance of Class A common stock upon exercise of stock options - - 908,679 - 7,685 - - 7,685 Stock-based compensation - - - - 13,772 - - 13,772 Exercise of Class A common stock warrants - - 113,116 - 9,220 - - 9,220 Net income - - - - - - 26,024 26,024 Balance at June 30, 2026 - $ - 46,826,550 $ - $ 787,321 $ ( 1,792 ) $ ( 248,723 ) $ 536,806 Six Months Ended June 30, 2025 Redeemable Convertible Preferred Stock Common Stock Additional Paid-In Capital Accumulated Other Comprehensive Loss Accumulated Deficit Total Stockholders' Deficit Shares Amount Shares Amount Balance at December 31, 2024 29,084,235 $ 419,409 10,925,950 $ - $ 30,545 $ - $ ( 282,201 ) $ ( 251,656 ) Issuance of common stock upon exercise of stock options - - 159,094 - 531 - - 531 Stock-based compensation - - - - 5,106 - - 5,106 Net loss - - - - - - ( 4,234 ) ( 4,234 ) Balance at June 30, 2025 29,084,235 $ 419,409 11,085,044 $ - $ 36,182 $ - $ ( 286,435 ) $ ( 250,253 ) The accompanying notes are an integral part of these interim financial statements. 6 Table of Contents BillionToOne, Inc. Statements of Cash Flows (in thousands, unaudited) Six Months Ended June 30, 2026 2025 Cash flows from operating activities: Net income (loss) $ 26,024 $ ( 4,234 ) Adjustments to reconcile net income (loss) to net cash provided by operating activities: Stock-based compensation 14,726 5,106 Depreciation and amortization 3,567 3,540 Amortization of operating right-of-use assets 2,819 2,455 Loss on disposal of fixed assets 64 3 Change in fair value of common stock warrant liability ( 62 ) ( 63 ) Change in fair value of redeemable convertible preferred stock warrant liability - 21 Change in fair value of term loan 3,805 591 Changes in operating assets and liabilities: Accounts receivable ( 33,277 ) ( 5,729 ) Inventories ( 3,590 ) ( 5,002 ) Prepaid expenses and other current assets ( 1,025 ) 2,417 Other non-current assets ( 566 ) ( 2,698 ) Accounts payable 4,866 3,313 Accrued expenses and other current liabilities 6,333 2,393 Accrued commissions ( 79 ) 216 Accrued compensation and employee benefits 4,495 4,207 Deferred revenue ( 1,150 ) ( 590 ) Operating lease liabilities ( 2,384 ) ( 2,139 ) Net cash provided by operating activities 24,566 3,807 Cash flows from investing activities: Purchases of property and equipment ( 8,520 ) ( 5,221 ) Net cash used in investing activities ( 8,520 ) ( 5,221 ) 7 Table of Contents BillionToOne, Inc. Statements of Cash Flows (in thousands, unaudited) Six Months Ended June 30, 2026 2025 Cash flows from financing activities: Proceeds from issuance of debt 30,000 - Principal payments on finance lease liabilities ( 301 ) ( 1,011 ) Payment of deferred offering costs ( 504 ) ( 625 ) Proceeds from exercise of stock options 7,412 531 Net cash provided by (used in) financing activities 36,607 ( 1,105 ) Net increase (decrease) in cash and cash equivalents 52,653 ( 2,519 ) Cash and cash equivalents at beginning of period 495,975 191,477 Cash and cash equivalents at end of period $ 548,628 $ 188,958 Supplemental cash flow disclosure: Cash payments for interest $ 3,442 $ 2,583 Cash paid for income taxes $ 173 $ 444 Supplemental non-cash investing and financing activities: Purchases of property and equipment in accounts payable and accrued expenses and other current liabilities $ 2,073 $ 1,365 Deferred offering costs in accounts payable and accrued expenses and other current liabilities $ - $ 1,916 Right-of-use assets obtained in exchange for new operating lease liabilities $ 4,010 $ - Cash paid for amounts included in the measurement of operating lease liabilities $ 4,647 $ 4,391 Net exercise of Class A common stock warrants $ 9,220 $ - Operating cash flows from financing leases (interest paid) $ 18 $ 68 Exercise of stock options for which cash had not been received $ 276 $ - The accompanying notes are an integral part of these interim financial statements. 8 Table of Contents BillionToOne, Inc. Notes to Unaudited Interim Financial Statements (1) Description of Business BillionToOne, Inc. (the “Company”) was formed in 2016 and is headquartered in Menlo Park, California. The Company is a precision diagnostics company that quantifies biology to create molecular diagnostics. The Company’s proprietary molecular counting platform is designed to detect and measure DNA molecules at the single-count level to help improve disease detection. The Company currently applies the proprietary technology to non-invasive prenatal screening (“Prenatal”) and liquid biopsy (“Oncology”). (2) Summary of Significant Accounting Policies Basis of Presentation The accompanying financial statements have been prepared in conformity with accounting principles generally accepted in the United States of America (“U.S. GAAP”). All intercompany transactions and balances have been eliminated upon consolidation. The unaudited interim financial statements should be read in conjunction with the audited financial statements and related notes for the year ended December 31, 2025 included in the Company’s Annual Report on Form 10-K filed with the SEC on March 11, 2026. For a summary of the Company’s significant accounting policies refer to “Note 2. Summary of Significant Accounting Policies” in the notes to the financial statements as of and for the year ended December 31, 2025 included in the Company’s Annual Report on Form 10-K. There have been no significant changes to these policies during the six months ended June 30, 2026. Unaudited interim financial information The unaudited financial statements do not include all disclosures, including certain notes required by GAAP on an annual reporting basis. The unaudited interim financial statements have been prepared on the same basis as the annual financial statements. In management’s opinion, the unaudited financial statements reflect all normal recurring adjustments necessary to state fairly the balance sheets, statements of operations and comprehensive income (loss), statements of redeemable convertible preferred stock and stockholders’ equity (deficit), and statements of cash flows for the interim periods, but are not necessarily indicative of the results of operations to be anticipated for the full fiscal year or any future period. Use of Estimates The preparation of financial statements in conformity with U.S. GAAP requires management to make estimates and assumptions that affect the reported amounts of assets and liabilities and disclosure of contingent assets and liabilities at the date of the financial statements and the reported amounts of revenues and expenses during the reporting period. On a regular basis, management evaluates estimates, including, but not limited to: stock-based compensation, deferred tax assets and liabilities, useful lives of long-lived assets, the incremental borrowing rate applied to operating and finance leases, determination of revenue recognition and accounts receivable, and valuation of debt and common stock warrants. These estimates are inherently subject to judgment and actual results could differ from those estimates. Risks and Uncertainties Certain of the Company’s product candidates are in development. If the Company is unable to advance its product candidates through development, clinical validation and ultimately commercialize its product candidates, or experiences significant delays in doing so, the Company’s business will be materially harmed. Even if the Company completes the necessary validation studies and product development, the process to meet any applicable regulatory requirements can be expensive. As a result, the Company cannot predict when, or if, it will be able to commercialize a product candidate. The Company is subject to certain risks and uncertainties that the Company believes could have a material adverse effect on its future financial position or results of operations. As of the date of issuance of the financial statements, the Company is not aware of any specific event or circumstance that would require it to update its estimates, judgments, or the carrying value of its assets or liabilities. These estimates may change as new 9 Table of Contents BillionToOne, Inc. Notes to Unaudited Interim Financial Statements events occur and additional information is obtained and are recognized in the financial statements as soon as they become known. Actual results could differ from those estimates, and any such differences may be material to the Company’s financial statements. The Company is subject to regulation and enforcement by the federal government and by authorities in state and foreign jurisdictions in which the Company conducts business. Such laws include, without limitation, state and federal anti-kickback, fraud and abuse, false claims, privacy and security laws and regulations. If the Company’s operations are found to be in violation of any such laws or government regulations that apply to use, the Company may be subject to penalties, including, without limitation, civil and criminal penalties, damages, fines, the curtailment or restructuring of operations and exclusion from participation in federal and state healthcare programs. Revenue The Company recognizes revenue upon transfer of control of promised goods and services in an amount that reflects the consideration it expects to be entitled to receive in exchange for those goods and services. Under ASC 606—Revenue from Contracts with Customers ("ASC 606"), the Company applies the following five-step approach: • Identify the contract with a customer • Identify the performance obligations in the contract • Determine the transaction price • Allocate the transaction price to the performance obligations in the contract • Recognize revenue when, or as, a performance obligation is satisfied The Company generates revenue primarily from prenatal and oncology testing services, which are referred to as testing services or test results. The Company considers the patient as its customer, that requests a test service through their physician. Test results are the single performance obligation being provided to customers. Testing service revenue is recognized at a point in time when test results are delivered to the ordering physician. The Company generally bills an insurance carrier, Medicaid, Medicare, the patient, or a combination upon delivery of test results. The Company enters into contracts with third-party payors, including insurance carriers, Medicaid and Medicare, to set the pricing for tests provided to patients. Due to the nature of these third-party payor contract arrangements, the total consideration the Company expects to collect for test results is variable as they are dependent on the terms negotiated with the third-party payor. The predominance of the Company’s revenue is derived from payments by third-party insurance carriers. The Company uses the expected value method of estimating variable consideration. The total consideration the Company expects to collect in exchange for the Company’s products is an estimate and is largely variable in nature. Consideration includes reimbursement from both patients and third-party payors. The Company establishes variable consideration by considering historical payment trends for tests delivered, test reimbursement disallowances, and contractual arrangements in place, among other factors, which is adjusted for current expectations. Current expectations of cash collections factor in changes in reimbursement rate trends, historical events not expected to recur, and future known changes such as anticipated contractual pricing changes or changes to insurance coverage. The Company also considers hindsight, where applicable, in estimates established for variable consideration and updates those estimates when actual experience supports doing so. In establishing variable consideration, the Company considers payors with similar reimbursement characteristics together. The Company monitors the cash collections against the estimated variable consideration over the expected cash collection period and any difference is recognized as an adjustment to estimated revenues after such estimated cash collection period has closed. In January 2023, the Company entered a partnership with Johnson & Johnson ("J&J") under which the Company is licensing the Company’s proprietary knowledge, performing clinical trial support services including developing a clinical study assay, and other testing services to support a clinical trial for the counterparty. In March 2026, the Company amended and restated the agreement governing the partnership with J&J to amend 10 Table of Contents BillionToOne, Inc. Notes to Unaudited Interim Financial Statements pricing and extend the term of the agreement, the extension is expected to have a duration of approximately three years . The Company concluded that the agreement and the amended agreement with J&J were within the scope of ASC 606 because the counterparty in the agreement meets the definition of a customer. The Company evaluated the terms of the agreement for revenue recognition, including whether the services are capable of being distinct and considered distinct within the context of the contract. The Company concluded that the licensing of the know how is not distinct from the other promises within the agreement and, as a result, was treated as a single performance obligation. Under this contract, the Company receives payments upon the achievement of milestones, including (i) receipt of approval of the trial, which was achieved in 2023, (ii) various patient enrollment milestones, and (iii) subsequent full trial completion, as well as reimbursement for testing services. In making an assessment of whether variable consideration should be included in the transaction price, the Company considers the degree of complexity and uncertainty associated with each milestone and related testing services, and whether achievement of the milestones and testing services are dependent on parties other than the Company. In July 2025, the Company entered into a partnership with J&J for the development and commercialization of a companion diagnostic (CDx), intended for use with a new drug candidate of J&J. The Company is providing services related to regulatory filings to support companion diagnostic submissions for the Company’s assay. The development and regulatory support services represent a single performance obligation as the Company performs a significant integration service, such as analytical validation and regulatory submissions. The individual promises are not separately identifiable from other promises in the contract, and therefore, not distinct. The Company receives payment from achievement of milestones, including (i) various CDx development milestones, (ii) FDA regulatory submission and pre-approval, and (iii) CDx approval by FDA. For the companion diagnostic development and regulatory approval performed, the Company is compensated through a combination of an upfront fee and performance-based, non-refundable regulatory and development milestones. The transaction price represents variable consideration and the Company uses the most likely amount to estimate variable consideration. Application of the constraint for variable consideration to milestone payments is an area that requires significant judgment. The Company evaluates factors such as the scientific, clinical, regulatory, and other risks that must be managed to achieve the respective milestone and the level of effort and investment required to achieve the respective milestone. In making this assessment, the Company considers its historical experience with similar milestones, the degree of complexity and uncertainty associated with each milestone, and whether the achievement of the milestone is dependent on parties other than the Company. The constraint for variable consideration is applied to the contract price such that it is probable a significant cumulative reversal of revenue will not occur when the uncertainty associated with the contingency is resolved. Application of the constraint for variable consideration is assessed and updated at each reporting period as a revision to the estimated transaction price. The Company recognizes revenue for the single performance obligation for each of the J&J partnership agreements over the period the services are provided. Specifically, the Company recognizes revenue using an input method to measure progress, utilizing costs incurred to-date relative to the total expected costs as a measure of progress. The Company assesses the changes to the total expected cost estimates in determining the revenue recognition for each reporting period. The Company applies the practical expedient not to disclose the value of unsatisfied performance obligations for contracts with an original expected length of one year or less. As of June 30, 2026, the Company’s remaining performance obligations beyond one year were approximately $ 6.2 million. 11 Table of Contents BillionToOne, Inc. Notes to Unaudited Interim Financial Statements Disaggregation of revenue The following table presents disaggregation of revenue by Prenatal, Oncology and Clinical trial support and other services for the three and six months ended June 30, 2026 and 2025 (in thousands): Three Months Ended June 30, Six Months Ended June 30, 2026 2025 2026 2025 Prenatal $ 94,248 $ 60,852 $ 190,773 $ 116,929 Oncology 13,663 4,947 24,383 7,123 Clinical trial support and other services 1,537 774 2,680 1,484 Total revenues $ 109,448 $ 66,573 $ 217,836 $ 125,536 Substantially all revenues recognized for the three and six months ended June 30, 2026 and 2025 were generated in the United States. Revenue related to performance obligations satisfied in prior periods For the three months ended June 30, 2026 and 2025, the Company recorded $ 2.8 million and $ 2.1 million, respectively, and for the six months ended June 30, 2026 and 2025, the Company recorded $ 12.0 million and $ 4.6 million, respectively, of revenue related to performance obligations satisfied in prior periods. In the first quarter of 2026, the Company entered into new agreements with payors that resulted in an increase of revenue related to expected payments on the reprocessing of claims through the date of the agreements, including claims from the year ended December 31, 2025. Revenue related to services performed during the year ended December 31, 2025 that were covered by these payors contributed slightly less than half of the $ 12.0 million in revenue related to performance obligations satisfied in prior periods for the six months ended June 30, 2026. Deferred revenue Deferred revenue, which is a contract liability, consists of billings or cash received for services in advance of revenue recognition and is recognized as revenue when all the Company’s revenue recognition criteria are met. As of June 30, 2026 and December 31, 2025, the deferred revenue balance was $ 2.3 million and $ 3.5 million, respectively. All deferred revenue as of June 30, 2026 was classified as current. As of December 31, 2025, $ 1.3 million of the deferred revenue balance was classified as non-current and $ 2.2 million was classified as current. For the three months ended June 30, 2026 and 2025, the Company recognized revenue from deferred revenue at the beginning of the period of $ 0.9 million and $ 0.8 million, respectively, and for the six months ended June 30, 2026 and 2025, recognized revenue from deferred revenue at the beginning of the period of $ 1.9 million and $ 1.5 million, respectively. Comprehensive Income (Loss) During the three and six months ended June 30, 2026 and 2025, the Company did not have any other comprehensive income (loss) and, therefore, the net income (loss) and comprehensive income (loss) was the same. Segment information Operating segments are defined as components of an entity for which separate financial information is available and that is regularly reviewed by the Chief Operating Decision Maker (“CODM”) in deciding how to allocate resources and assess performance. The Company’s Chief Executive Officer is the Company’s CODM. The CODM reviews financial information presented on a company-wide basis to make operating decisions, allocating resources, and evaluating financial performance. As such, the Company has determined that it is managed as one operating segment and one reportable segment. Key areas of focus for the CODM when making decisions on the allocations of resources is cash used in operations as well as revenue, gross margin and net income (loss) and comprehensive income (loss); this information is used by the CODM and compared to budgeted amounts in order for the CODM to make decisions 12 Table of Contents BillionToOne, Inc. Notes to Unaudited Interim Financial Statements on how resources should be allocated across the organization. The Company’s segment measure of profitability is net income (loss) and comprehensive income (loss). Segment revenues are predominantly derived from prenatal and oncology testing results, leveraging the Company’s diagnostic technology platform, which are delivered to patients, who are the Company’s customer. The Company’s customers are predominantly located in the United States. Substantially all of the Company’s long-lived assets are located in the United States. The Company’s technology platform is applied similarly in both the prenatal and oncology settings. The financial statements provide the CODM with a view of the Company’s financial condition as it pertains to the Company’s assets, liabilities and expenses. Significant expense categories align with the expense categories and amounts presented on the statements of operations and comprehensive income (loss). Recently Adopted Accounting Pronouncements In July 2025, the FASB issued ASU 2025-05, Measurement of Credit Losses for Accounts Receivable and Contract Assets , which provides for a practical expedient to estimate credit losses related to accounts receivable and contract assets from revenue contracts accounted for in accordance with ASC 606 using information as of the balance sheet date. The Company adopted this ASU effective for the Company’s fiscal year beginning January 1, 2026 and it did not have a material impact on the Company’s financial statements and related disclosures. Recently Issued Accounting Pronouncements not yet Adopted In November 2024 and January 2025, the FASB issued ASU 2024-03 and ASU 2025-01, respectively, Income Statement—Reporting Comprehensive Income—Expense Disaggregation Disclosures (Subtopic 220-40): Disaggregation of Income Statement Expenses , which requires disclosure in the notes to the financial statements of specified information about an entity’s certain costs and expenses. The amendments to the standards are effective for the Company’s fiscal year beginning January 1, 2027 and interim periods beginning January 1, 2028. Early adoption is permitted. The amendments should be applied either prospectively to the financial statements issued for reporting periods after the effective date of this ASU or retrospectively to any or all prior periods presented in the financial statements. The Company is currently evaluating this ASU to determine the impact it may have on its financial statements and related disclosures. In September 2025, the FASB issued ASU 2025-06, Intangibles-Goodwill and Other-Internal-Use Software (Subtopic 350-40): Targeted Improvements to the Accounting for Internal-Use Software , which clarifies and expands the existing guidance on capitalizing implementation costs for cloud computing arrangements that are service contracts. The new guidance is effective for public business entities for fiscal years beginning after December 15, 2027, and interim periods within those fiscal years. Early adoption is permitted. The Company is currently evaluating this ASU to determine the impact it may have on its financial statements and related disclosures. In September 2025, the FASB issued ASU 2025-07, Derivatives Scope Refinements and Scope Clarification for Share-Based Noncash Consideration from a Customer in a Revenue Contract , which amends the guidance under ASC Topic 815 (Derivatives and Hedging) and ASC Topic 606 (Revenue from Contracts with Customers). The ASU (i) refines the scope for derivative accounting to exclude certain non-exchange-traded contracts whose underlyings are based on the operations or activities specific to one of the parties to the contract, and (ii) provides clarification on how to account for share-based noncash consideration from customers (such as equity instruments, warrants, or shares) received in exchange for the transfer of goods or services under a revenue contract. The new guidance is effective for public business entities for fiscal years beginning after December 15, 2026, and interim periods within those fiscal years. Early adoption is permitted. The Company is currently evaluating this ASU to determine the impact it may have on its financial statements and related disclosures. 13 Table of Contents BillionToOne, Inc. Notes to Unaudited Interim Financial Statements (3) Fair Value Measurements The fair value measurements of assets and liabilities that are measured at fair value on a recurring basis consisted of the following as of June 30, 2026 (in thousands): As of June 30, 2026 Level 1 Level 2 Level 3 Total Current assets: Money market funds $ 424,595 $ - $ - $ 424,595 Total assets $ 424,595 $ - $ - $ 424,595 Liabilities: Term loan $ - $ - $ 91,031 $ 91,031 Total liabilities $ - $ - $ 91,031 $ 91,031 The fair value measurements of assets and liabilities that are measured at fair value on a recurring basis consisted of the following as of December 31, 2025 (in thousands): As of December 31, 2025 Level 1 Level 2 Level 3 Total Current assets: Money market funds $ 359,813 $ - $ - $ 359,813 Total assets $ 359,813 $ - $ - $ 359,813 Liabilities: Common stock warrants $ - $ 9,282 $ - $ 9,282 Term loan - - 57,226 57,226 Total liabilities $ - $ 9,282 $ 57,226 $ 66,508 Level 3 instruments consist of the Company’s term loan due to the lack of relevant observable market data for the respective fair value inputs of the instrument. In August 2024, the Company entered into a term loan agreement which the Company elected to account for using the fair value option. As such, the fair value of the debt is calculated by using the probability weighting of the present value of settlement scenarios (See Note 7). The significant assumptions used in preparing the income approach model for valuing the term loan as of June 30, 2026 and December 31, 2025, are as follows: June 30, 2026 December 31, 2025 Discount rate 6.80 % 7.10 % Annual interest rate 8.00 % 8.00 % Expected term (years) – Scenario 1 3.75 4.25 Expected term (years) – Scenario 2 5.1 5.6 The fair values of the Class A common stock warrants are measured using an option pricing model. The common stock warrant liability was reclassified to equity upon the exercise of the underlying warrants; as such, the common stock warrant liability fair value was determined as of the date of exercise, on May 6, 2026 (see Note 7). The fair value of the warrants on the date of exercise is equal to the intrinsic value, as the expected term is reduced to zero . The intrinsic value was determined based on the closing price of the 14 Table of Contents BillionToOne, Inc. Notes to Unaudited Interim Financial Statements Company’s Class A common stock on the date of exercise of $ 81.40 , less the exercise price of each of the warrants of $ 2.80 and $ 10.92 . The significant assumptions used in preparing the option pricing model for valuing the common stock warrant liability as of December 31, 2025 are as follows: December 31, 2025 Exercise price $ 2.80 – $ 10.92 Volatility 53.5 % Expected term (years) 0.3 Risk-free rate 3.63 % Dividend yield - % The redeemable convertible preferred stock warrant liability was reclassified to equity upon the exercise of the underlying warrants; as such, the redeemable convertible preferred stock warrant liability fair value was determined as of the date of exercise, on November 14, 2025. The following tables present a summary of the changes in the fair value of the Company’s Level 3 financial instruments (in thousands): Term Loan Balance at December 31, 2025 $ 57,226 Additions 30,000 Adjustments to fair value 2,779 Balance at March 31, 2026 90,005 Adjustments to fair value 1,026 Balance at June 30, 2026 $ 91,031 Common Stock Warrants Redeemable Convertible Preferred Stock Warrants Term Loan Balance at December 31, 2024 $ 2,533 $ 230 $ 51,481 Adjustments to fair value ( 58 ) 2 1,797 Balance at March 31, 2025 2,475 232 53,278 Adjustments to fair value ( 5 ) 19 ( 1,206 ) Balance at June 30, 2025 $ 2,470 $ 251 $ 52,072 For the three and six months ended June 30, 2026 and 2025, the Company recognized losses related to the change in the fair value of the term loan in change in fair value of term loan in the statements of operations and comprehensive income (loss). For the three and six months ended June 30, 2026 and 2025, the Company recognized gains and losses related to the change in the fair value of the common stock warrant liability in other income (expense), net in the statements of operations and comprehensive income (loss). For the three and six months ended June 30, 2025, the Company recognized losses related to the change in convertible preferred stock warrant liability in other income (expense), net in the statements of operations and comprehensive income (loss). 15 Table of Contents BillionToOne, Inc. Notes to Unaudited Interim Financial Statements The common stock warrant liability is recorded within common stock warrant liability on the balance sheet as of December 31, 2025. The term loan is recorded within long-term debt on the balance sheets as of June 30, 2026 and December 31, 2025. During the three and six months ended June 30, 2026 and 2025, the Company had no transfers of financial assets or liabilities between different levels of the fair value hierarchy. (4) Accrued Expenses and Other Current Liabilities Accrued expenses and other current liabilities consisted of the following (in thousands): June 30, 2026 December 31, 2025 Accrued professional services $ 1,862 $ 2,591 Accrued inventory purchases 5,292 995 Accrued fixed asset purchases 610 1,006 Accrued property and other taxes 150 188 Accrued information technology purchases 628 462 Accrued legal expense 1,322 968 Accrued phlebotomy expense 413 200 Accrued insurance expense 149 — Accrued rent 965 174 Accrued clinical expenses 169 35 Other 1,043 628 Accrued expenses and other current liabilities $ 12,603 $ 7,247 (5) Commitments and Contingencies Legal Proceedings From time to time, the Company is party to certain claims in the ordinary course of business. The Company, in conjunction with its legal counsel, assesses the need to record a liability for litigation or contingencies. A liability is recorded when and if it is determined that such a liability for litigation or contingencies is both probable and the amount can be reasonably estimated. The Company believes that it is not presently a party to any litigation, other than as described below, the outcome of which, if determined adversely, would individually or in the aggregate be expected to have a material and adverse effect on the business, operating results, cash flows, or financial position. Legal fees are expensed in the period in which they are incurred. Illumina, Inc. v. BillionToOne, Inc. On May 7, 2026, Illumina, Inc. filed a lawsuit in the U.S. District Court in the State of Delaware alleging that the Company’s UNITY test infringes U.S. Patent Nos. 10,612,096, 12,139,760 and 12,435,373. The Company maintains that Illumina’s allegations are without merit, and is vigorously defending itself against this claim. The Company does not believe that a loss is probable or reasonably estimable related to this matter. For more information regarding risks relating to our intellectual property, see Part I, Item 1A, 'Risk Factors,' of the Company's Annual Report on Form 10-K for the fiscal year ended December 31, 2025. Indemnification Agreements The Company has entered into indemnification agreements with its directors and officers against any liabilities that may arise by reason of their status or service as directors or officers, other than liabilities arising from willful misconduct of the individual. Additionally, in the ordinary course of business, the Company enters into agreements of varying scope and terms pursuant to which it agrees to indemnify customers, vendors, lessors, business partners, and other 16 Table of Contents BillionToOne, Inc. Notes to Unaudited Interim Financial Statements parties with respect to certain matters, including, but not limited to, losses arising out of the breach of such agreements, services to be provided by the Company, or from intellectual property infringement claims made by third parties. As of June 30, 2026 and December 31, 2025, no demands have been made upon the Company to provide indemnification under such agreements, and there are no claims that the Company is aware of that could have a material adverse effect on its financial position, results of operations, or cash flows. Accordingly, the Company has no liabilities recorded for these agreements as of June 30, 2026 and December 31, 2025. (6) Leases Operating Leases In June 2026, the Company entered into a lease agreement for office and laboratory space in Union City, CA. The lease is expected to commence in the second half of 2027. The lease agreement provides for a rent abatement period of twelve months following the commencement date, during which no base rent will be due. Following the abatement period, base rent will commence at an initial annual rate of $ 3.6 million per year with annual increases of 3 %. The lease has a term of twelve years from the commencement date. The agreement also includes a ten-year extension option that can be exercised by the Company. As part of the lease agreement, the Company is required to remit to the landlord $ 0.4 million in respect of a security deposit and $ 0.3 million in respect of the first month’s rent payment. (7) Long-term Debt Oberland Note Purchase Agreement In August 2024, the Company entered into a note purchase agreement (the “2024 Notes”) with BWCB SA LLC, an entity affiliated with Oberland Capital Management, LLC (“Oberland Capital”), which provided the Company with up to four tranches of capital advances totaling up to $ 140.0 million. The advanced principal accrues interest at a rate of 8.0 % per annum. The first tranche of $ 50.0 million was advanced on August 5, 2024, with a Maturity Date on the seventh anniversary of the first purchase date (August 5, 2031). The first tranche requires interest-only payments through August 5, 2031 and a lump sum payment due on August 5, 2031. The second tranche of up to $ 35.0 million in principal was available at the Company’s option at any time prior to September 30, 2025 provided that the trailing six-month worldwide net revenue of the Company is at least $ 80.0 million. The Company did not elect the option to draw on the second tranche. The Company was required to sell the third tranche of notes in the amount of $ 30.0 million prior to March 31, 2026 as the Company achieved the revenue and gross margin thresholds triggering this obligation during the first half of fiscal 2025. The third tranche of $ 30.0 million was advanced on March 31, 2026, with a Maturity Date on the seventh anniversary of the initial purchase date (August 5, 2031). The third tranche requires interest-only payments through August 5, 2031 and a lump sum payment due on August 5, 2031. The thresholds triggering this tranche were trailing six-month revenue of at least $ 112.5 million and a trailing six-month gross margin of at least 45 %. Gross Margin is defined as (I) net revenue minus cost of goods sold divided by (II) net revenue, expressed as a percentage. The terms of the third tranche are identical to those of the first $ 50.0 million tranche. Lastly, the fourth tranche of up to $ 25.0 million in principal was made available to the Company at the mutual agreement of the parties at any time prior to March 31, 2026. The Company did not elect the option to draw on the fourth tranche. The Company has the option at any time to prepay all of the then-outstanding notes, and Oberland Capital has the option to redeem the notes upon (i) a change in control of the Company, (ii) an event of default, or (iii) the maturity date. The redemption price of the notes shall equal to the following: (1) 130 % of principal amounts of notes if the payment is made within 24 months of issuance; (2) 145 % of principal amounts of notes if the payment is made within 36 months of issuance; (3) If the payment is made within 48 months, an amount that would generate an internal rate of return (“IRR”) of 12.25 %; (4) if the payment is made within 60 months of the issuance, an amount that would generate an IRR of 11.75 %; (5) if the payment is made thereafter but before 17 Table of Contents BillionToOne, Inc. Notes to Unaudited Interim Financial Statements maturity, an amount that would generate an IRR of 11.25 %; and (6) if the payment is made at maturity, an amount that would generate an IRR of 10.0 %. The Company is required to maintain trailing six-month net revenue based on a schedule that gradually increases up to $ 120.0 million as of the year ending December 31, 2026. Period Minimum Trailing Six-Month Net Revenue Threshold (in millions) Q1 2026 $ 87.2 Q2 2026 $ 101.8 Q3 2026 $ 117.2 Q4 2026 $ 120.0 Thereafter $ 120.0 In addition, the Company is required to maintain a trailing six-month Gross Margin of not less than 30 %. The agreement also contains a revenue participation provision, under which, for any fiscal quarter, 0.01 % of net revenue for such fiscal quarter (up to $ 100.0 million of net revenue for each fiscal year) per each $ 1.0 million principal amount of the notes will be payable to Oberland Capital. Amounts paid under the revenue participation provision during the three and six months ended June 30, 2026 and 2025 were interest payments on the debt. The revenue participation payments are additional financing costs of the loan and are included in the computation of the internal rate of return measures described above and do not reduce principal on the debt. During the three months ended June 30, 2026 and 2025, the Company made revenue participation payments of $ 0.3 million and $ 0.2 million, respectively, and during the six months ended June 30, 2026 and 2025, the Company made revenue participation payments of $ 0.8 million and $ 0.5 million, respectively. The Company elected to account for the 2024 Notes using the fair value option and changes in fair value related to the 2024 Notes are recorded in change in fair value of term loan on the Company’s statements of operations and comprehensive income (loss). The Company also elected to present interest incurred on the 2024 Notes in the change in fair value of the term loan; interest expense under the 2024 Notes was $ 1.6 million and $ 1.0 million for the three months ended June 30, 2026 and 2025, respectively, and $ 2.6 million and $ 2.0 million for the six months ended June 30, 2026 and 2025, respectively. Total debt outstanding as of June 30, 2026 and December 31, 2025 was $ 91.0 million and $ 57.2 million, respectively, and included within long-term debt on the Company’s balance sheets. Future principal payments of the Company’s long-term debt as of June 30, 2026 and December 31, 2025 are $ 80.0 million and $ 50.0 million, respectively, and are due during the year ending December 31, 2031. The term loan advances are secured by a lien on the Company’s assets. Western Alliance Bank Debt In October 2021, the Company entered into a loan and security agreement (the “2021 LSA”) with Western Alliance Bank (“WAB”), which provided the Company with three tranches of capital advances totaling $ 15.0 million. In July 2022, the Company amended the 2021 LSA, such that WAB made four tranches of capital advances available to the Company for an aggregate amount up to $ 35.0 million (the "2022 LSA Amendment"). During August 2024, the Company elected to prepay the outstanding amount of the term loans of $ 35.0 million in principal and the $ 1.5 million exit fee that became payable upon early loan payoff. Common Stock Warrants In connection with the 2021 LSA, the Company issued to Western Alliance Bank (“WAB”) warrants to purchase shares of the Company’s common stock at an exercise price of $ 2.80 per share. The number of underlying shares of the warrants was initially 53,571 and was increased to 80,357 upon the funding of the loans in January 2022. The warrants were to expire if unexercised on October 12, 2031. Upon the occurrence of an acquisition of the Company, if the acquiror shall not have assumed the warrants, WAB shall have the right to put 18 Table of Contents BillionToOne, Inc. Notes to Unaudited Interim Financial Statements the warrants back to the Company for cash equal to the greater of (x) $ 450,000 or (y) the value of the aggregate consideration payable to WAB had WAB exercised the warrants immediately prior to exercise such put right. In connection with the 2022 LSA Amendment, the Company issued up to 41,209 warrants for common stock at an exercise price of $ 10.92 per share to WAB, 30,907 warrants were exercisable upon execution of the agreement; the remaining warrants became exercisable as the Company made additional draws on the 2022 LSA Amendment. As of December 31, 2025 all of the warrants were exercisable. The warrants were to expire if unexercised on July 22, 2032. Upon the occurrence of an acquisition of the Company, if the acquiror shall not have assumed the warrants, WAB shall have the right to put the warrants back to the Company for cash equal to the greater of (x) $ 450,000 or (y) the value of the aggregate consideration payable to WAB had WAB exercised the warrants immediately prior to exercise such put right. All the warrants issued to WAB were puttable warrants and thus were liability classified. The warrants were initially recognized at fair value with any subsequent changes in fair value to be recorded in other income (expense), net in the statements of operations and comprehensive income (loss) (See Note 3). In connection with the Company’s IPO, WAB entered into a lock-up agreement with the Company, pursuant to which WAB may not sell, transfer, or otherwise dispose of the underlying shares of Class A common stock for a period of 180 days following the date that the Company filed its final prospectus. Following the expiration of the lock-up agreement, in May 2026, the Class A common stock warrants were net exercised, resulting in the issuance of 113,116 shares of Class A common stock, with 8,450 shares withheld to cover the aggregate exercise price. (8) Common Stock As of June 30, 2026 and December 31, 2025, the Company’s Certificate of Incorporation, as amended, authorized the Company to issue 800,000,000 shares of Class A common stock, par value $ 0.00001 per share, and 10,000,000 shares of Class B Common Stock, par value $ 0.00001 per share. Total common stock outstanding as of June 30, 2026 and December 31, 2025 was 42,283,900 and 41,252,105 shares of Class A common stock, respectively, and 4,542,650 and 4,552,650 shares of Class B common stock, respectively. The holders of Class A common stock are entitled to vote on all corporate matters at a ratio of one vote per share together with the holders of Class B common stock. The holders of Class B common stock are entitled to vote on all corporate matters at a ratio of 15 votes per share together with the holders of Class A common stock. The holders of Class A common stock and Class B common stock are also entitled to receive dividends whenever funds are legally available and when and if declared by the Board of Directors, subject to the prior rights of holders of all series of stock outstanding. As of June 30, 2026 and December 31, 2025, no dividends had been declared or paid. All of the outstanding shares of the Company’s Class B common stock will convert automatically on a one -to-one basis into shares of the Company’s Class A common stock upon the earliest of (i) 7 years from the date of filing the Post-IPO Certificate of Incorporation, and (ii) the date specified by a vote of the holders of Class B common stock representing a majority of the outstanding shares of Class B common stock. In addition, each share of Class B common stock held by a Co-Founder and such Co-Founder's permitted transferees will automatically convert into one share of Class A common stock upon the earlier of (i) the date that is between 90 days and 270 days, as determined by the Board of Directors, after the death or incapacitation of such Co-Founder or (ii) the date that is between 61 and 180 days, as determined by the Board of Directors, after the date on which such Co-Founder is no longer serving as an officer or director of the Company. During the six months ended June 30, 2026, 10,000 shares of Class B common stock were converted into an equivalent number of shares of Class A common stock at the option of the holder. The conversion did not impact the total number of shares of common stock outstanding. The Company is required to reserve and keep available out of its authorized but unissued shares of Class A common stock such a number of shares sufficient to affect the conversion of all outstanding shares of common stock warrants, all outstanding shares of Class B common stock, options granted under the Company’s 2018 Stock Plan and 2025 Equity Incentive Plan, shares available for grant under the Company’s 2025 Equity 19 Table of Contents BillionToOne, Inc. Notes to Unaudited Interim Financial Statements Incentive Plan, restricted stock units outstanding and shares available for issuance under the 2025 Employee Stock Purchase Plan. The amount of such shares of the Company’s Class A common stock reserved for these purposes at June 30, 2026 is as follows: Outstanding shares of Class B common stock 4,542,650 Options to purchase common stock 8,896,341 Restricted stock units outstanding 229,300 Shares available for future issuance under the Employee Stock Purchase Plan 548,880 Shares available for future grants 2,907,999 Total common stock reserved 17,125,170 Stock Plan In December 2018, the Company adopted the 2018 Stock Plan (the “2018 Plan”). The 2018 Plan authorizes the granting of stock options upon the approval of the Company’s Board of Directors, to employees and consultants providing services to the Company. Stock options granted under the 2018 Plan generally expire within 10 years from the date of grant and are generally issued at the fair value of the underlying shares of common stock on the date of grant as determined by the Company’s Board of Directors. The shares subject to each option typically allow for 25 % of the shares to vest and become exercisable on the first anniversary of the vesting commencement date and thereafter, the remaining 75 % will vest and become exercisable in 36 equal monthly installments. The Company may include other vesting terms from time to time. Incentive and non-statutory stock options may be granted with exercise prices not less than 100 % of the estimated fair value of the common stock on the date of grant. In connection with the IPO, the Company adopted the 2025 Equity Incentive Plan (the “2025 Plan”), which became effective in October 2025, although no awards could be granted under the 2025 Plan until the IPO date. The 2018 Plan was terminated upon the completion of the IPO, however, awards outstanding under the 2018 Plan will remain outstanding and will continue to be governed by their existing terms. On the effective date of the 2025 Plan, the maximum number of shares of Class A common stock authorized for issuance was 3,297,972 shares of Class A common stock, plus up to 10,034,626 shares under the 2018 Plan that (i) remain available for issuance on the IPO date and (ii) that are outstanding on the IPO date and that are subsequently forfeited, expire, are reacquired by the Company or lapse unexercised, subject to annual adjustment on the first day of each fiscal year, beginning January 1, 2027, through January 1, 2035. The 2025 Plan authorizes the granting of stock options and restricted stock units, among other types of awards, to employees and consultants providing services to the Company. Stock options granted under the 2025 Plan generally expire within 10 years from the date of grant. Stock options and restricted stock units are issued based on the fair value of the underlying shares of Class A common stock on the date of grant. The stock options and restricted stock units vest in 8 semi-annual installments each equal to 12.5 % of the total shares under the grant or one initial installment of 25 % of the total shares under the grant on the first anniversary of the grant date and, thereafter, in 6 semi-annual installments equal to 12.5 % of the total shares under the grant. The Company may include other vesting terms from time to time. Incentive and non-statutory stock options may be granted with exercise prices not less than 100 % of the fair value of the Class A common stock on the date of grant, as determined by the Board of Directors. As of June 30, 2026 and December 31, 2025, the Company had authorized up to 3,489,011 and 3,390,306 shares of Class A common stock reserved for issuance under the 2025 Plan, respectively. In October 2025, the Company adopted the 2025 Employee Stock Purchase Plan (the “2025 ESPP”), which became effective in October 2025. As of June 30, 2026, the number of shares of Class A common stock available for issuance under the 2025 ESPP is 548,880 shares of Class A common stock, subject to annual adjustment on the first day of each fiscal year, beginning January 1, 2027, through January 1, 2035. No more than 8,400,000 shares of Class A common stock may be issued under the 2025 ESPP. Each participant may purchase up to the number of shares determined by the Board of Directors on any purchase date, not to exceed 20 Table of Contents BillionToOne, Inc. Notes to Unaudited Interim Financial Statements 7,500 shares. The price of each share of Class A common stock purchased under the 2025 ESPP will not be less than 85 % of the lower of the fair market value per share of Class A common stock on the first day of the applicable offering period or the fair market value per share of Class A common stock on the purchase date. The 2025 ESPP provides for separate six-month offering periods beginning on June 22 and December 22 of each year. The grant date fair value of the stock purchase rights granted under the 2025 ESPP was estimated on the first day of each offering period using the Black-Scholes option pricing model. The valuation methodology used was substantially consistent with that used to value stock options with the exception of the expected term, which was based on the term of each offering period, and related impacts on expected volatility and risk-free interest rate. Stock-based Compensation Stock Option Activity The weighted average assumptions used to calculate the fair value of option grants issued under the 2025 Plan and 2018 Plan during the three and six months ended June 30, 2026 and 2025 were as follows: Three Months Ended June 30, Six Months Ended June 30, 2026 2025 2026 2025 Dividend yield - % - % - % - % Risk-free interest rate 4.3 % 4.2 % 4.2 % 4.2 % Expected volatility 73 % 74 % 74 % 73 % Expected term (in years) 6.14 6.36 6.15 6.31 A summary of the Company’s stock option activity and related information is as follows: Number of Options Outstanding Weighted Average Exercise Price Per Share Aggregate Intrinsic Value (in thousands) Balance as of December 31, 2025 9,552,013 $ 12.96 $ 657,915 Granted 355,036 $ 84.11 Exercised ( 908,679 ) $ 8.46 Forfeited or expired ( 102,029 ) $ 18.53 Balance as of June 30, 2026 8,896,341 $ 16.28 $ 922,461 Vested and exercisable as of June 30, 2026 4,803,993 $ 7.66 $ 539,580 Vested and expected to vest as of June 30, 2026 8,896,341 $ 16.28 $ 922,461 As of June 30, 2026, there was approximately $ 85.2 million of total unrecognized compensation cost related to unvested options, which is expected to be recognized over the weighted average period of 3.6 years. Restricted Stock Unit Activity A summary of the Company’s restricted stock unit activity and related information is as follows: 21 Table of Contents BillionToOne, Inc. Notes to Unaudited Interim Financial Statements Shares Weighted- Average Grant Date Fair Value (per share) Nonvested as of January 1, 2026 33,332 $ 108.94 Issued 197,499 74.61 Vested - - Forfeited ( 1,531 ) 79.03 Nonvested as of June 30, 2026 229,300 $ 77.10 As of June 30, 2026, there was approximately $ 19.2 million of total unrecognized compensation cost related to outstanding restricted stock units, which is expected to be recognized over the weighted average period of 3.4 years. There was no restricted stock unit activity during the three and six months ended June 30, 2025. ESPP Activity The grant date fair value of the stock purchase rights granted under the 2025 ESPP for the three and six months ended June 30, 2026 were as follows: Three Months Ended June 30, Six Months Ended June 30, 2026 2026 Dividend yield - % - % Risk-free interest rate 4.0 % 4.0 % Expected volatility 66 % 66 % Expected term (in years) 0.50 0.50 As of June 30, 2026, there was approximately $ 1.2 million of total unrecognized compensation cost related to stock purchase rights granted under the 2025 ESPP, which is expected to be recognized over the weighted average period of 0.5 years. Stock-Based Compensation Expense The table below shows stock-based compensation expense included in the statements of operations and comprehensive income (loss) for the three and six months ended June 30, 2026 and 2025 (in thousands): Three Months Ended June 30, Six Months Ended June 30, 2026 2025 2026 2025 Cost of revenue $ 787 $ 386 $ 1,421 $ 739 Research and development 1,753 823 3,109 1,571 Selling, general and administrative 5,677 1,523 10,196 2,796 Total stock-based compensation $ 8,217 $ 2,732 $ 14,726 $ 5,106 (9) Income Taxes Accounting for income taxes for interim periods generally requires the provision for income taxes to be determined by applying an estimate of the annual effective tax rate for the full fiscal year to income or loss before income taxes, adjusted for discrete items, if any, for the reporting period. The Company updates its estimate of the annual effective tax rate each quarter and makes a cumulative adjustment in such period. The Company recorded income tax benefit of $ 0.5 million and income tax expense of $ 14.5 thousand for the three months ended June 30, 2026 and 2025, respectively, and the Company recorded income tax expense of $ 2.6 22 Table of Contents BillionToOne, Inc. Notes to Unaudited Interim Financial Statements thousand and $ 0.1 million for the six months ended June 30, 2026 and 2025, respectively. Income tax expense (benefit) consists primarily of income taxes for U.S. federal and the states in which the Company conducts business and the excess tax benefit from the stock based compensation. Due to the Company’s history of losses in the United States, a full valuation allowance on substantially all of the Company’s deferred tax assets, including net operating loss carryforwards, research and development tax credits, and other book versus tax differences, was maintained. Given the Company's current earnings and anticipated future earnings, the Company believes that there is a reasonable possibility that sufficient positive evidence may become available in the near term to allow the Company to reach a conclusion that a significant portion of the valuation allowance will no longer be needed. Any release of the valuation allowance will result in a material benefit recognized in the quarter of release. A release of the valuation allowance would result in the recognition of an increase in deferred tax assets and an income tax benefit in the period in which the release occurs, although the exact timing and amount of the release is subject to change based on numerous factors, including projections of future taxable income, which continues to be assessed based on available information each reporting period. (10) Employee Benefit Plan The Company sponsors a qualified 401(k) defined contribution plan covering eligible employees. Participants may contribute a portion of their annual compensation limited to a maximum annual amount set by the Internal Revenue Service. Employer contributions to the plan are discretionary. During the three months ended June 30, 2026 and 2025, the Company contributed $ 1.2 million and $ 0.9 million to this plan, respectively, and during the six months ended June 30, 2026 and 2025, the Company contributed $ 2.3 million and $ 1.7 million to this plan, respectively. (11) Related Party Transactions In June 2025, the Company granted stock options to certain founders and executive officers under the Company’s 2018 Plan. These grants were approved by the Board of Directors. There were no material related party transactions during the three and six months ended June 30, 2026. (12) Net Income (Loss) Per Share The Company applies the two-class method when computing net income (loss) per share attributable to common shareholders when shares meet the definition of participating securities. The two-class method determines net income (loss) per share of common stock and participating securities according to dividends declared or accumulated and participation rights in undistributed earnings. The two-class method requires net income (loss) available to common stockholders for the period to be allocated between common stock and participating securities based upon their respective rights to receive dividends as if all income for the period had been distributed. The holders of the Company’s redeemable convertible preferred stock were entitled to dividends in preference to common stockholders, if declared. Such dividends were not cumulative. Any remaining earnings would be distributed among the holders of redeemable convertible preferred stock and common stock pro rata on an as-converted basis. The holders of the Company’s redeemable convertible preferred stock were not contractually obligated to participate in the Company’s losses. Basic net income (loss) per share is computed by dividing the net income (loss) attributable to common stockholders by the weighted-average number of shares of common stock outstanding during the period, less shares subject to repurchase. The dilutive effect of potentially dilutive common shares is reflected in diluted earnings per share by application of the if-converted method for the Company’s outstanding preferred stock, the treasury stock method for the Company’s other potentially dilutive securities, and is ultimately applied by sequencing instruments beginning with those that are more dilutive. For periods in which the Company reports net losses, diluted net income (loss) per common share is the same as basic net income (loss) per common share as all potentially dilutive securities are anti-dilutive. 23 Table of Contents BillionToOne, Inc. Notes to Unaudited Interim Financial Statements The following table sets forth the computation of basic and dilutive net income (loss) per share attributable to common stockholders for the three and six months ended June 30, 2026 and 2025 (in thousands, except per share amounts): Three Months Ended June 30, Six Months Ended June 30, 2026 2025 2026 2025 Basic net income (loss) per share: Numerator: Net income (loss) $ 8,054 $ ( 246 ) $ 26,024 $ ( 4,234 ) Denominator: Weighted-average shares used in calculating net income (loss) per share, basic 46,403,350 10,385,000 46,169,335 10,349,146 Net income (loss) per share, basic $ 0.17 $ ( 0.02 ) $ 0.56 $ ( 0.41 ) Diluted net income (loss) per share: Numerator: Net income (loss) $ 8,054 $ ( 246 ) $ 26,024 $ ( 4,234 ) Denominator: Weighted-average shares used in calculating net income (loss) per share, basic 46,403,350 10,385,000 46,169,335 10,349,146 Effect of dilutive securities: Outstanding stock options and other 8,008,597 — 7,495,459 — Warrants to purchase common stock 43,576 — 78,447 — Weighted-average shares used in calculating net income (loss) per share, diluted 54,455,523 10,385,000 53,743,241 10,349,146 Net income (loss) per share, diluted $ 0.15 $ ( 0.02 ) $ 0.48 $ ( 0.41 ) Potentially dilutive securities that were not included in the diluted per share calculations because they would be anti-dilutive were as follows for the three and six months ended June 30, 2026 and 2025: Three Months Ended June 30, Six Months Ended June 30, 2026 2025 2026 2025 Redeemable convertible preferred stock - 29,084,235 - 29,084,235 Outstanding stock options 13,874 8,933,419 6,937 8,933,419 Restricted stock awards in exchange for non-recourse note - 700,000 - 700,000 Warrants to purchase common stock - 121,566 - 121,566 Warrants to purchase redeemable convertible preferred stock - 9,660 - 9,660 (13) Subsequent Events In July 2026, the Company entered into an agreement with a third party under which the third party will perform research and development services over an approximately eight-month period. Under the agreement, the Company will pay a fee of $ 3.0 million, which the Company will expense as the services are performed over the service period. 24 Table of Contents