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Item 1. Business

2026-02-27 compared with 2025-03-03 · 242 added, 299 removed, 288 unchanged (65% of the section changed)

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Our technology platforms include magnetic resonance, mass spectrometry, gas and liquid chromatography, X-ray, microscopy, metrology, and molecular spectroscopy technologies.

Bruker is enabling innovation, improved productivity, and customer success in post-genomic life science molecular and cell biology research and offers differentiated, high value life science and diagnostics systems and solutions in preclinical imaging, clinical phenomics research, proteomics and multiomics, spatial and single-cell biology, functional structural and condensate biology, as well as in clinical microbiology and molecular diagnostics.

We are enabling innovation, improved productivity, and customer success in post-genomic life science molecular and cell biology research and offer differentiated, high value life science and diagnostics systems and solutions in preclinical imaging, clinical phenomics research, proteomics and multiomics, spatial and single-cell biology, functional structural and condensate biology, as well as in clinical microbiology and molecular diagnostics.

Our corporate headquarters are located in Billerica, Massachusetts.

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Business Segments

We have four reportable segments, Bruker Scientific Instruments (BSI) BioSpin, BSI CALID (Chemicals, Applied Markets, Life Science, In Vitro Diagnostics, Detection), BSI NANO, and Bruker Energy & Supercon Technologies (BEST).

Our products, which have particular application in structural proteomics, drug discovery, pharmaceutical and biotechnology research and production, and the food and materials science fields, provide customers with the ability to determine the structure, dynamics, and function of specific molecules, such as proteins, and thus allows them to understand fundamental biological processes including the formation and progression of diseases.

Furthermore, BSI BioSpin’s product enables the characterization of mixtures and also complex materials, rendering them high value-added tools for a variety of industrial applications.

We have four reportable segments, Bruker Scientific Instruments (“BSI”) BioSpin, BSI CALID (Chemicals, Applied Markets, Life Science, In Vitro Diagnostics, Detection), BSI NANO, and Bruker Energy & Supercon Technologies (“BEST”), which each comprise several divisions as disclosed below.

Our products, which have a particular application in structural proteomics, drug discovery, pharmaceutical and biotechnology research and production, and the food and materials science fields, primarily provide customers with the ability to determine the structure, dynamics, and function of specific molecules, such as proteins, and thus allows them to understand fundamental biological processes including the formation and progression of diseases.

BSI BioSpin Segment

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The majority of BSI BioSpin’s customers are academic and government research facilities.

Other customers include pharmaceutical and biotechnology companies;

battery, chemical, food and beverage, clinical and polymer companies;

and nonprofit laboratories.

During 2024, we continued our focus on accelerating growth, enhancing customer relationships, and driving innovation with our key initiatives, which include expanding into high-potential markets, leveraging core strengths, and driving recurring revenue through aftermarket and connected services.

With three additional successful installations of 1.2 GHz NMR systems, we continue to advance ultra-high field access, supporting studies in structural biology, pharmacology, and cellular biology, aligning with Bruker's mission to provide cutting-edge technology for scientific research.

Furthermore, during 2024, we completed our acquisition of Spectral Instruments Imaging and Chemspeed Technologies AG, and the minority investment in NovAliX, which align with the segment's goals of expanding its technological capabilities, entering new markets, and enhancing its product portfolio.

The acquisition of Spectral Instruments Imaging, a leader in preclinical in-vivo optical imaging systems, augments in the technology and product portfolio of the Bruker BioSpin Preclinical Imaging division, broadening its range of preclinical solutions for disease research.

Additionally, the acquisition of Chemspeed Technologies AG provides us entry into lab automation and digitalization, complementing our existing product platform of vendor-agnostic solutions.

Through the minority investment in NovAliX, we have taken a step into the preclinical Contract Research Organization (CRO) space, specializing in drug discovery services.

This partnership aims to advance our expertise in structural biology and biophysics.

Other customers include pharmaceutical and biotechnology companies, battery, chemical, food and beverage, clinical and polymer companies, and nonprofit laboratories.

During 2025, we continued our focus on enhancing customer relationships and driving innovation with our key initiatives, which include expanding into high-potential markets, leveraging core strengths, and driving recurring revenue through aftermarket and connected services.

With two additional successful installations of 1.2 GHz NMR systems, we continue to advance ultra-high field access, supporting studies in structural biology, pharmacology, and cellular biology, aligning with Bruker's mission to provide cutting-edge technology for scientific research.

Furthermore, during 2025, we completed certain minority investments which align with the segment’s goals of expanding its technological capabilities, entering new markets, and enhancing its product portfolio.

BSI BioSpin Segment’s instruments are based on the following technology platforms:

NMR—Nuclear magnetic resonance

Qualitative & quantitative analytical technique to determine molecular structure & purity of sample.

Molecules placed in a magnetic field, give off radio frequency signature recorded by a detector.

Analysis software helps determine molecular structure.

Used in Academia by pharmaceutical, biotechnology, food and beverage & clinical companies, and other industrial users in life science and material science research.

EPR—Electron paramagnetic resonance

A process for absorption of microwave radiation by paramagnetic ions or molecules with at least one unpaired electron that spins in the presence of static magnetic field.

This technique detects unpaired electrons unambiguously, whereas other techniques only provide indirect evidence of presence.

This technique can identify the paramagnetic species detected, presenting information on molecular structure & giving insight into dynamic processes such as molecular motions or fluidity.

Used in advanced materials research, materials analysis, quality control.

MRI—Magnetic resonance imaging

Process of creating image from manipulation of hydrogen atoms in a magnetic field.

In the presence of an external magnetic field, atoms align with or against it.

The application of a radio frequency causes atoms to jump between high and low energy states.

MRI and magnetic resonance spectroscopy (“MRS”) include different methods, such as:

diffusion-weighted, perfusion-weighted, molecular imaging and contrast-enhance.

It offers high resolution morphologic information, functional, metabolic or molecular information.

Used in pharmaceutical research, including metabolomics, to study a number of diseases including diabetes, neurology, oncology, and cardiovascular disorders.

MPI—Magnetic particle imaging

Process of creating an image from magnetic particles administered to the body of an animal.

Magnetic particles are manipulated in a combination of oscillating magnetic fields exhibiting a field free zone.

Response of the particles allows real time 3D data set acquisition of the whole body of an animal, showing the contrast agent distributing in and flowing through the body.

Used to detect cardiovascular disorders.

PET—Positron emission tomography

Process of creating an image from positrons after administration of a positron emitting radionuclide to the body of an animal.

Annihilation of the positron produces two photons which show an angle of 180° between them, distinguishing these photons from photons originating from other sources.

Tracer enriches in certain regions of interest within the body and gains molecular information from the animal in vivo.

Used in oncology, inflammation, neurology, and cardiovascular disorders, as well as metabolic disease, drug discovery, and bone diseases.

SPECT—Single photon emission tomography

Uses a contrast agent containing radionuclides which directly emit single photons.

Contrast agent enriches in certain parts of the body of an animal and generates images of the radionuclide distribution in the body.

Used in animal investigation in vivo, most importantly in oncology, neurology, and cardiovascular disorders.

CT—Computed tomography

Technology based on X-rays which are used to generate a complete 3D data set.

Offers highest spatial resolution of all preclinical imaging modalities and is especially useful to generate morphological information about the object or animal under investigation.

Used in tissue sample analysis or non-invasive in vivo animal imaging.

Used in wide range of preclinical investigations in the fields of bone-orthopedics, cardiology, pulmonology, oncology, and metabolism among others.

Automation—Flexible lab automation solutions

• Software —Comprehensive data management suite.

NMR is a qualitative and quantitative analytical technique that is used to determine the molecular structure and purity of a sample.

Molecules are placed in a magnetic field and give off a radio frequency signature that is recorded by a sensitive detector.

Analysis software helps to determine the molecular structure of the sample.

The NMR technique is used in academia, by pharmaceutical, biotechnology, food and beverage and clinical companies, and by other industrial users in life science and material science research.

EPR is a process of absorption of microwave radiation by paramagnetic ions or molecules with at least one unpaired electron that spins in the presence of a static magnetic field.

EPR detects unpaired electrons unambiguously, whereas other techniques can only provide indirect evidence of their presence.

In addition, EPR can identify the paramagnetic species that are detected, which present information on the molecular structure near the unpaired electron and give insight into dynamic processes such as molecular motions or fluidity.

Our EPR instruments are used for a wide range of applications, including advanced materials research, materials analysis and quality control.

MRI is a process of creating an image from the manipulation of hydrogen atoms in a magnetic field.

In the presence of an external magnetic field, atoms will align with or against the external magnetic field.

Application of a radio frequency causes the atoms to jump between high and low energy states.

MRI and magnetic resonance spectroscopy, or MRS, include many methods including diffusion-weighted, perfusion-weighted, molecular imaging and contrast-enhance.

MRI offers high resolution morphologic information, as well as functional, metabolic or molecular information.

Customers use our MRI systems in pharmaceutical research, including metabolomics, to study a number of diseases, including diabetes, neurology, oncology and cardiovascular disorders.

MPI is a process of creating an image from magnetic particles administered to the body of an animal.

The magnetic particles are manipulated in a combination of oscillating magnetic fields exhibiting a field free zone.

The response of the particles allows a real time 3D data set acquisition of the whole body of an animal, showing the contrast agent distributing in and flowing through the body.

This imaging modality is used to detect cardiovascular disorders.

PET is a process of creating an image from positrons after administration of a positron emitting radionuclide to the body of an animal.

Annihilation of the positron produces two photons which show an angle of 180° between them, distinguishing these photons from photons originating from other sources.

The PET tracer enriches in certain regions of interest within the body and gains

molecular information from the animal in vivo .

This has widespread applications, most importantly for oncology, inflammation, neurology and cardiovascular disorders, as well as metabolic disease, drug discovery and bone disease.

SPECT uses a contrast agent containing radionuclides which directly emit single photons.

The contrast agent enriches in certain parts of the body of an animal and generates images of the radionuclide distribution in the body.

SPECT has widespread application in animal investigations in vivo , most importantly in oncology, neurology and cardiovascular disorders.

CT is a technology based on X-rays which are used to generate a complete 3D data set.

The most important applications are tissue sample analysis or non-invasive in vivo animal imaging.

CT offers the highest spatial resolution of all preclinical imaging modalities and is especially useful to generate morphological information about the object or animal under investigation.

CT is being used in a wide range of preclinical investigations in the fields of bone-orthopedics, cardiology, pulmonology, oncology and metabolism among others.

The Automation portfolio offers platforms and digital tools for research and development and quality control labs, supporting various applications including synthesis, NMR, XRD, and IR.

These solutions are designed for scalability, modularity, and flexibility, enhancing lab connectivity, saving time and costs, and boosting outcomes.

Offers platforms and digital tools for research and development and quality control labs, supporting various applications including synthesis, nuclear magnetic resonance, X-ray diffraction, and infrared spectroscopy.

These applications are designed for scalability, modularity, and flexibility, enhancing lab connectivity, saving time and costs, and boosting outcomes.

Rooted in scientific expertise, the technology provides compliance-ready and configurable systems to fit exact workflows.

The comprehensive Software suite of data management solutions is designed to facilitate digitalization and readiness for artificial intelligence and automation.

It includes tools for experiment design, data analysis, and process management, enabling real-time understanding, monitoring, and control of processes.

Additionally, it integrates robotics and automation technologies to provide transformative solutions, supporting the automation and digitalization of labs and factories under a unified platform.

Used in research and development and quality control labs.

Software—Comprehensive data management suite

Designed to facilitate digitalization and readiness for artificial intelligence and automation.

Includes tools for experiment design, data analysis, and process management, enabling real-time understanding, monitoring, and control of processes.

This program also integrates robotics and automation technologies to provide transformative solutions, supporting the automation and digitalization of labs and factories under a unified platform.

Used to support the automation and digitalization of labs and factories under a unified platform.

BLI—Bioluminescence imaging

Non-invasive in-vivo imaging technique enables real-time monitoring of biological processes within living organisms utilizing light-emitting molecules, such as luciferase, which produce signals detectable by highly specialized optical imaging systems.

The DNA encoding the luminescent protein is incorporated into the animal either via a viral vector or by creating a transgenic animal.

Used in oncology, immunotherapy, and infectious disease research to study disease dynamics, monitor the effects of therapies, and assess cellular behaviors in live animal models.

FLI—Fluorescence imaging

Non-invasive imaging technique that uses fluorescent molecules to detect and visualize biological processes in living organisms.

When exposed to specific wavelengths of excitation light, these molecules emit light at different wavelengths, which can be captured by specialized optical imaging systems.

Used in oncology, immunotherapy, and infectious disease research for cell tracking, biodistribution of a drug delivery system, and multi-target/multi-plex applications.

The BSI BioSpin Segment also offers a range of services, product lifecycle support, scientific software, and workflow solutions to customers who use BSI BioSpin products.

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Our Genotype and Fluorotype molecular diagnostics (“MDx”) kits enable a culture-free detection and analysis of microbes and viruses directly from patient samples with a special focus on tuberculosis, HIV viral load, viral hepatitis and sexually transmitted diseases.

Molecular Diagnostics utilize PCR assays and systems to provide diagnostic solutions for a number of different disease states, including respiratory, mycobacteria (including tuberculosis), virology, safety of immunocompromised patients, sexually transmitted infections, gastroenteric diseases as well as other microbiology tests.

Molecular Diagnostics utilize PCR assays and systems to provide diagnostic solutions

for a number of different disease states, including respiratory, mycobacteria (including tuberculosis), virology, safety of immunocompromised patients, sexually transmitted infections, gastroenteric diseases as well as other microbiology tests.

Depending on the assay being used, the technology enables users to ascertain basic identification of a certain infection, distinguish infections which can cause similar symptoms and detect specific microbial resistance, all from a single sample.

Our portfolio includes FluoroType ® , using fluorescence-based real-time PCR technology, and more recently we have also developed LiquidArray ® assays based on melt curve analysis for optimized asymmetrical PCR technology.

LiquidArray ® uses light-on-off probes, providing a powerful technology to identify a broad number of indicators for different infections or

resistance markers from a single sample, providing greater depth of information.

Following the acquisition of ELITechGroup, our portfolio now includes InGenius and BeGenius systems which are integrated Sample-to-Answer PCR systems that perform automated nucleic acid extraction, specific PCR reaction and data interpretation without user interaction.

LiquidArray ® uses light-on-off probes, providing a powerful technology to identify a broad number of indicators for different infections or resistance markers from a single sample, providing greater depth of information.

Following the acquisition of ELITechGroup in 2024, our portfolio now includes InGenius and BeGenius systems which are integrated sample-to-answer PCR systems that perform automated nucleic acid extraction, specific PCR reaction and data interpretation without user interaction.

• Bruker Optics:

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These products are utilized in industry, government, and academia for a wide range of applications and solutions for life science, pharmaceutical, food and agricultural analysis, quality control, and process analysis applications.

Infrared and Raman spectroscopies are widely used in both research and industry as simple, rapid, nondestructive and reliable techniques for applications ranging from basic sample identification and quality control to advanced research and also in remote sensing setups for environmental control.

Infrared and Raman spectroscopies are widely used in both remote sensing setups for environmental control, as well as research and industry as simple, rapid, nondestructive, and reliable techniques for applications ranging from basic sample identification and quality control to advanced research.

The technologies and instruments of the division are also used for military and civil purposes in the field of detection of chemical, biological, radioactive, nuclear substances and explosives (“CBRNE”).

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Customers of our BSI CALID Segment include pharmaceutical, biotechnology and diagnostics companies, contract research organizations, academic institutions, medical schools, nonprofit or for-profit forensic laboratories, agriculture, food and beverage safety, environmental and clinical microbiology laboratories, hospitals, and government departments and agencies.

During 2024, we continued our focus on accelerating growth, enhancing customer relationships, and driving innovation through the launch of our new NIR-spectrometer BEAM, the first dedicated single-point spectrometer utilizing the full power of FT-NIR spectroscopy as well as introducing our next-generation version of its Multi-Purpose Analyzer (MPA-III).

We also introduced several other new technologies and workflows such as Novor V2.0, a machine learning algorithm trained on HLA peptides for immunopeptidomics;

TwinScape a cloud-based liquid chromatography and mass spectrometry quality control application;

lyco-PASEF® a glycopeptide analysis mode;

a discovery service for deep plasma proteomics, with optional integration of Alamar Bio's NULISA® panels for inflammation and CNS studies;

and we released the neoFLEX benchtop MALDI TOF for spatial biology application and the timsTOF Ultra 2 mass spectrometer, with enhanced sensitivity for low input and single cell applications.

Furthermore, during 2024, we completed our acquisition of Tornado Spectral Systems Inc.

(Toronto, Canada), Nanophoton Corporation (Osaka, Japan), Dynamic Biosensors GmbH (Munich, Germany and Massachusetts, U.S.A.) as well as ELITechGroup in various locations.

The acquisition of Tornado Spectral Systems Inc., a Canadian company specializing in process Raman instruments, complemented the segments Raman spectrometers products with its patented High-Throughput Virtual Slit (HTVS) technology and Raman analyzers such as the HyperFlux PRO Plus, Process Guardian and SuperFlux for more accurate chemical identification and quantification in mixtures and at low concentrations.

The acquisition of Nanophoton Corporation augmented our Bruker Optics division with its broad portfolio of advanced Raman microscopes, capable of reducing measurement time by several hundred times and employing a unique beam-scanning method based on stochastic process and information theories.

The acquisition of Dynamic Biosensors GmbH expanded our biophysical analytics portfolio including measurement of molecular interactions and kinetics.

The acquisition of ELITechGroup which is active in the molecular diagnostics, microbiology and biomedical testing equipment field, complemented the segments MALDI BioTyper® platform for bacterial and fungal identification and establish Bruker as an innovative and growing infectious disease specialist in the IVD market.

During 2025, we continued our focus on enhancing customer relationships and driving innovation through the launch of our MOVE-T solution for the dairy markets as well as the launch of the VERTEX NEO R FTIR research spectrometer.

We also introduced several other new technologies and workflows advancing proteomics and multiomics in Mass Spectrometry such as:

timsOmni a novel instrument type which ushers an era of functional proteomics and proteoformanalysis;

timsMetabo a benchtop 4D-Metabolomics instrument for unprecedented annotation confidence with high sensitivity;

the timsUltra Athena Ion Processor (“AIP”) our instrument for ultra-high sensitivity 4D single-cell proteomics and immunopeptidomics based on the AIP technology;

and the proteoElute our new nanoflow liquid chromatography system for robust, ultra-sensitive proteomics with real-time monitoring.

Furthermore, during 2025, we completed our acquisition of Recipe Chemicals + Instruments GmbH (“Recipe”), AST Revolution, LLC, as well as certain other acquisitions and minority investments.

The acquisition of Recipe, a provider of vendor-agnostic therapeutic drug monitoring and other clinical in vitro diagnostic kits for Liquid chromatography-mass spectrometry systems utilizing triple-quadrupole time-of-flight mass spectrometry, High Performance Liquid Chromatography, and Inductively coupled plasma mass spectrometry assays, enhances our capabilities in small molecule clinical diagnostic assays for our liquid chromatography triple-quadrupole mass spectrometers.

The acquisition of AST Revolution, LLC, an in vitro diagnostics company supporting rapid antimicrobial susceptibility testing, further enhances and complements our product portfolio.

The BSI CALID Segment’s instruments are based on the following technology platforms:

• MALDI-TOF —Matrix-assisted laser desorption ionization time-of-flight mass spectrometry, including tandem time-of-flight systems (MALDI-TOF);

• ESI-TOF —Electrospray ionization time-of-flight spectrometry, including trapped ion mobility (TIMS) based on ESI-quadrupole-TOF mass spectrometry (timsTOF);

• MRMS —Magnetic resonance mass spectrometry, including hybrid systems with a quadrupole front end (Q-q-MRMS);

• ITMS —Ion trap mass spectrometry;

• IMS – Ion mobility spectrometry;

• GC-MS —Gas chromatography-mass spectrometry systems utilizing triple-quadrupole time-of-flight mass spectrometry;

• LC-MS —Liquid chromatography-mass spectrometry systems utilizing triple-quadrupole time-of flight mass spectrometry;

• FT-IR —Fourier transform-infrared spectroscopy;

• NIR —Near-infrared spectroscopy;

• PCR —Polymerase chain reaction;

• Raman —Raman spectroscopy and microscopy, and

• QCL IR – Quantum Cascade LASER Infrared spectroscopy and microscopy.

MALDI-TOF mass spectrometers utilize an ionization process to analyze solid samples using a laser that combines high sample throughput with high mass range and sensitivity.

Our MALDI-TOF mass spectrometers are particularly useful for applications in clinical diagnostics, environmental and taxonomical research and food processing and quality control.

MALDI-TOF—Matrix-assisted laser desorption ionization time-of-flight mass spectrometry, including tandem time-of-flight systems

Mass spectrometers utilize an ionization process to analyze solid samples using a laser that combines high sample throughput with high mass range and sensitivity.

Allows users to classify and identify microorganisms quickly and reliably with minimal sample preparation efforts and life cycle costs.

Useful for applications in clinical diagnostics, environmental and taxonomical research , and food processing and quality control.

Specific applications include:

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and fast body fluid and tissue peptide or protein biomarker detection.

MALDI mass spectrometry allows users to classify and identify microorganisms quickly and reliably with minimal sample preparation efforts and life cycle costs.

Our MALDI BioTyper solution, which serves the clinical microbiology market, enables identification, taxonomical classification or dereplication of microorganisms like bacteria, yeasts and fungi.

ESI-TOF mass spectrometers utilize an electrospray ionization process to analyze liquid samples.

This ionization process, which does not dissociate the molecules, allows for rapid data acquisition and analysis of large biological molecules as well as complex biosamples.

ESI-TOF mass spectrometers are particularly useful for:

identification, protein analysis and functional complex analysis in proteomics and protein function;

Serves the clinical microbiology market, enables identification, taxonomical classification or dereplication of microorganisms like bacteria, yeasts and fungi.

ESI-TOF—Electrospray ionization time-of-flight spectrometry, including trapped ion mobility (“TIMS”) based on ESI-quadrupole-TOF mass spectrometry (“timsTOF”)

Mass spectrometers utilize an electrospray ionization process to analyze liquid samples.

This ionization process, which does not dissociate the molecules, allows for rapid data acquisition and analysis of large biological molecules and complex biosamples.

Useful for identification, protein analysis and functional complex analysis in proteomics and protein function;

molecular identification in metabolomics, natural product and drug metabolite analysis;

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and fast liquid chromatography mass spectrometry, or liquid chromatography mass spectrometry ( “ LC-MS ” ), in drug discovery and development.

MRMS systems utilize high-field superconducting magnets to offer the highest resolution, selectivity, and mass accuracy currently achievable in mass spectrometry.

MRMS—Magnetic resonance mass spectrometry, including hybrid systems with a quadrupole front end (“Q-q-MRMS”)

Utilize high-field superconducting magnets to offer the highest resolution, selectivity, and mass accuracy currently achievable in mass spectrometry.

Our systems based on this technology often eliminate the need for time-consuming separation techniques in complex mixture analyses.

In addition, our systems can fragment molecular ions to perform exact mass analysis on all fragments to determine molecular structure.

MRMS systems are particularly useful for:

the study of the structure and function of biomolecules, including proteins, DNA and natural products;

We offer next-generation hybrid MRMS systems that combine a traditional external quadrupole mass selector and hexapole collision cell with a high-performance MRMS for further ion dissociation, top-down proteomics tools and ultra high-resolution detection.

Useful for the study of the structure and function of biomolecules, including proteins, DNA and natural products;

complex mixture analysis including body fluids or combinatorial libraries;

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and top-down proteomics of intact proteins without the need for enzymatic digestion of the proteins prior to analysis.

IMS systems utilize Ion-mobility spectrometry (IMS) is an analytical technique used to separate and identify ionized molecules in the gas phase based on their mobility in a carrier buffer gas.

Though heavily employed for military or security purposes, such as detecting chemical warfare agents and explosives (explosive trace detection, ETD), the technique also has many laboratory and analytical applications.

ITMS systems collect all ions simultaneously, which improves sensitivity relative to previous quadrupole mass spectrometers.

Ion trap mass spectrometers are particularly useful for sequencing and identification based on peptide structural analysis, quantitative liquid chromatography mass spectrometry, identification of combinatorial libraries and generally enhancing the speed and efficiency of the drug discovery and development process.

GC-MS systems combine the features of gas chromatography and mass spectrometry to identify different substances within a test sample.

ITMS—Ion trap mass spectrometry

Collects all ions simultaneously, which improves sensitivity relative to previous quadrupole mass spectrometers.

Useful for sequencing and identification based on peptide structural analysis, quantitative liquid chromatography mass spectrometry, identification of combinatorial libraries, and generally enhancing the speed and efficiency of the drug discovery and development process.

IMS—Ion mobility spectrometry

Analytical technique used to separate and identify ionized molecules in the gas phase based on their mobility in a carrier buffer gas.

Heavily employed for military or security purposes, such as detecting chemical warfare agents and explosives (explosive trace detection, “ETD”).

Also has many laboratory and analytical applications.

GC-MS—Gas chromatography-mass spectrometry systems utilizing triple-quadrupole time-of-flight mass spectrometry

Combines the features of gas chromatography and mass spectrometry to identify different substances within a test sample.

The two components, used together, allow for a finer degree of substance identification than either system when used separately.

The result is a quantitative analysis of the components and the mass spectrum of each component.

Our GC-MS systems are available in triple quadrupole configurations and can be configured with a variety of options to suit a range of applications.

Our GC-MS systems have applications in food and product safety, forensics, clinical and toxicology testing and environmental, pharmaceutical and chemical analysis.

LC-MS systems combine the separation features of liquid chromatography with the molecular identification features of mass spectrometry to separate, identify and quantify different substances within a test sample.

Available in triple quadrupole configurations and can be configured with a variety of options to suit a range of applications.

Used in applications in food and product safety, forensics, clinical and toxicology testing and environmental, pharmaceutical, and chemical analysis.

DART-MS—Direct Analysis in Real Time mass spectrometer system utilizing triple quadrupole and time of flight spectrometry

API technique that enables rapid, chromatography-free analysis of solids, liquids, and gases in their native form, with virtually no sample preparation.

Uses a heated gas stream and plasma to generate excited-state species that ionize analytes directly for mass spectrometry results in seconds.

This source technology can be integrated into existing mass spectrometry systems or deployed as a fully automated solution, supporting high-throughput workflows such as 96-well plate screening in 25 minutes or less.

Applications span forensics, clinical research, food, pharmaceuticals, environmental, and industrial, offering flexibility and speed at the point of need.

LC-MS—Liquid chromatography-mass spectrometry systems utilizing triple-quadrupole time-of flight mass spectrometry

Combines the separation features of liquid chromatography with the molecular identification features of mass spectrometry to separate, identify and quantify different substances within a test sample.

As a complementary technique to GC-MS, which analyzes volatile compounds, LC-MS can be used to analyze a wide range of non-volatile compounds in complex samples.

Our LC-MS systems are available in a wide range of configurations to suit a user’s specific needs.

Although primarily used for life science applications, our LC-MS systems also have applications in food and product safety, forensics and clinical and toxicology testing, as well as environmental, pharmaceutical and chemical analysis.

FT-IR spectrometers utilize the mid- and far-infrared regions of the electromagnetic spectrum.

Our FT-IR systems are commonly used for various quality control and materials research applications.

NIR spectrometers utilize the near-infrared region of the electromagnetic spectrum.

Our NIR instruments are primarily used for quality and process control applications in the pharmaceutical, food and agriculture and chemical industries.

These systems are available in a wide range of configurations to suit a user’s specific needs.

Primarily used for life science applications, also has applications in food and product safety, forensics and clinical and toxicology testing, as well as environmental, pharmaceutical and chemical analysis.

FT-IR—Fourier transform-infrared spectroscopy

Spectrometers utilize the mid- and far-infrared regions of the electromagnetic spectrum.

Used for various quality control and materials research applications.

NIR—Near-infrared spectroscopy

Spectrometers utilize the near-infrared region of the electromagnetic spectrum.

Used for quality and process control applications in the pharmaceutical, food and agriculture, and chemical industries.

The pharmaceutical industry is the leading user of NIR instruments, and applications include quality control, research and development, and process analytical technology.

The food and agricultural industry is the second largest user of NIR instrumentation, with an increasing demand for food, feed and beverage quality control.

PCR t he innovative LiquidArray ® technology optimizes asymmetrical multiplex PCR for creating excess single-stranded amplicons with detection by lights-on/-off probes that contain a quencher (lights-off) or both fluorophore and quencher (lights-on).

PCR—Polymerase chain reaction

The innovative LiquidArray ® technology optimizes asymmetrical multiplex PCR for creating excess single-stranded amplicons with detection by lights-on/-off probes that contain a quencher (lights-off) or both fluorophore and quencher (lights-on).

During melting curve analysis, lights-on/-off probes detach from the amplicon at specific temperatures and as fluorescence is either emitted or suppressed, specific fluorescence signatures are generated by the unique FluoroCycler ® XT thermocycler for the LiquidArray ® multiplex PCR technology.

The LiquidArray ® technology supports multiplexed assays where a large number of targets is analyzed simultaneously from single samples.

The LiquidArray ® technology supports multiplexed assays where a large number of targets are analyzed simultaneously from single samples.

For example, the LiquidArray ® -powered, WHO-endorsed FluoroType ® MTDBR VER 2.0 assay detects more than 500 genotypes by the combined analysis of up to 45 different mutations in mycobacteria.

Raman spectroscopy provides information on molecular structure.

Used in clinical microbiology and infectious diseases testing, for example, for syndromic panel testing of gastro-intestinal and sexually transmitted diseases.

Additionally, assays are used for the detection of tuberculosis infections, viral load testing of HIV patients, and for the monitoring of transplant patients.

Raman—Raman spectroscopy and microscopy

Provides information on molecular structure.

The mechanism of Raman scattering is different from that of infrared absorption, in that Raman and IR spectra provide complementary information.

Raman is useful for the identification of both organic and inorganic compounds and functional groups.

It is a nondestructive technique and can be used for the analysis of both liquids and solids.

Raman is well suited for use in the polymer and pharmaceutical industries, and has applications in the metals, electronics and semiconductors industries.

Useful for the identification of both organic and inorganic compounds and functional groups.

Nondestructive technique that can be used for the analysis of both liquids and solids.

It is also well suited for use in the polymer and pharmaceutical industries and has applications in the metals, electronics and semiconductors industries.

The technique also has applications in life sciences, forensics, and artwork authentication.

QCL IR spectroscopy utilizes a different source for generating Infrared (IR) light, which is a quantum cascade LASER which constitutes a tunable (mid-)IR LASER.

QCL IR—Quantum Cascade LASER Infrared spectroscopy and microscopy

Utilizes a different source for generating infrared (“IR”) light, which is a quantum cascade LASER which constitutes a tunable mid-IR LASER.

Quantum cascade lasers are fundamentally different from the conventional thermal sources which are used for FT-IR.

QCL exhibits a spectral power density which is typically orders of magnitudes higher than that of a thermal source, therefore providing advantages in terms of applicability of samples and speed of measurement particularly for microscopy or imaging experiments.

This technique is applied in life sciences, forensics, semiconductor industries and others.

Used in life sciences, forensics, semiconductor industries and others.

Additionally, the Bruker Detection product line offers a wide range of portable analytical and bioanalytical detection systems and related products for CBRNE detection.

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The BSI NANO Segment comprises of the following:

• The Bruker AXS Division :

• Bruker AXS :

Designs, manufactures, and distributes advanced X-ray instruments that use electromagnetic radiation with extremely short wavelengths to determine the characteristics of matter and the three-dimensional structure of molecules.

This includes a product portfolio of instruments based on X-ray fluorescence spectroscopy (XRF), X-ray diffraction (XRD) and X-ray micro computed tomography (µCT), or X-ray microscopy, as well as spark optical emission spectroscopy systems (S-OES) used to analyze the concentration of elements in metallic samples.

• The Bruker Nano Analytics Division:

Manufactures and markets analytical tools for electron microscopes, including energy-dispersive X-ray spectrometers (EDS), electron backscatter diffraction systems (EBSD) and µCT accessories, as well as mobile and bench top micro X-ray fluorescence (µXRF), total reflection X-ray fluorescence spectrometers (TXRF) and handheld, portable and mobile X-ray fluorescence (HMP-XRF) spectrometry instruments.

• The Bruker Nano Surfaces and Metrology Division:

The product portfolio includes instruments based on X-ray fluorescence (“XRF”), micro-X-ray fluorescence (“µXRF”) and total reflection X-ray fluorescence (“TXRF”) spectroscopy, X-ray diffraction (“XRD”), X-ray micro computed tomography (“µCT”), also called X-ray microscopy, as well as spark optical emission spectroscopy systems (“OES”) used to analyze the concentration of elements in metallic samples.

The Bruker AXS Division also offers high-end scanning

transmission electron microscopes (“STEM”) and a range of analytical tools for electron microscopes, including energy-dispersive X-ray spectrometers (“EDS”) and electron backscatter diffraction systems (“EBSD”).

• Bruker Nano Surfaces and Metrology:

This division's products include atomic force microscopy instrumentation (“AFM).

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In addition, the division manufacturers and markets automated X-ray metrology, automated AFM defect-detection, and photomask repair and cleaning equipment for semiconductor process control.

• Bruker Spatial Biology Division:

Provides the CosMx Spatial Molecular Imager and GeoMx Digital Spatial Profiler technologies for interrogating spatial transcriptomics, the nCounter technology for quantitation of gene expression, and the Cellscape technology for precision spatial proteomics.

These technologies allow researchers to elucidate gene and protein expression in a spatial context, which is useful for characterizing the underlying biology of organs and tissues, as well as for deriving deep biological insight into gene and protein expression for the development of biomarkers.

• Bruker Spatial Biology:

Provides the CosMx Spatial Molecular Imager and GeoMx Digital Spatial Profiler technologies for interrogating spatial transcriptomics, the nCounter technology for quantitation of gene expression, the Cellscape technology for precision spatial proteomics, and the PaintScape technology for single-cell 3D visualization of genome architecture and organization.

These technologies allow researchers to elucidate genomic structure and gene and protein expression in a spatial context, which is useful for characterizing the underlying biology of organs and tissues, as well as for deriving deep biological insight for the development of biomarkers.

In addition, Bruker Spatial Biology offers a variety of services for transcriptional profiling and multiomic analysis, spanning the spectrum from early discovery research to translational research and clinical trials.

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Customers of our BSI NANO Segment include academic institutions, governmental customers, nanotechnology companies, semiconductor companies, raw material manufacturers, industrial companies, biotechnology and pharmaceutical companies, and other businesses involved in materials analysis.

During 2024 we acquired substantially all of the assets and rights associated with NanoString Technologies, a leading provider of life-science research solutions for spatial transcriptomics and gene expression analysis.

We subsequently created the Bruker Spatial Biology division, as described above, consisting of NanoString Technologies and Canopy Biosciences, along with Bruker Spatial Genomics.

Furthermore, during 2024, we acquired Nion LLC, a manufacturer of innovative high-end scanning transmission electron microscopes, enhancing Bruker’s product offerings in materials science research and providing the technology base for applications in electron diffraction crystallography.

Subsequently the Nion operations were integrated into the Bruker AXS division.

During 2025, we completed certain minority investments which align with the segment’s goals of expanding its technological capabilities, entering new markets, and enhancing its product portfolio.

The BSI NANO Segment systems are based on the following technology platforms:

XRD—Polycrystalline X-ray diffraction, often referred to as X-ray diffraction

• XRF —X-ray fluorescence, also called X-ray spectrometry, including handheld XRF systems;

• SC-XRD— Single crystal X-ray diffraction, often referred to as X-ray crystallography;

• µCT— X-ray micro computed tomography, X-ray microscopy;

• EDS— Energy dispersive X-ray spectroscopy on electron microscopes;

• EBSD— Electron backscatter diffraction on electron microscopes;

• S-OES— Spark optical emission spectroscopy;

• CS/ONH— Combustion analysis for carbon, sulfur, oxygen, nitrogen, and hydrogen in solids;

• STEM —scanning transmission electron microscopes

• AFM —Atomic force microscopy;

• FM —Fluorescence microscopy;

• SOM —Stylus and optical metrology;

• TMT —Tribology and mechanical test systems for analysis of friction and wear;

• NanoIR —Nanoscale infrared spectroscopy;

• Alicona —Focus variation optical technology for non-contact dimensional metrology;

• BCA —Optofluidic platforms and Proteomic Barcoding platforms;

• Canopy —Multiplexed fluorescence-based single cell imaging as well as multi-omics sample characterization;

• NanoString – platforms for spatial transcriptomic analysis and for the measurement of gene expression through detection of RNA abundance

XRD systems investigate polycrystalline samples or thin films with single wavelength X-rays.

Investigate polycrystalline samples or thin films with single wavelength X-rays.

The atoms in the polycrystalline sample scatter the X-rays to create a unique diffraction pattern recorded by a detector.

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Our XRD systems contribute to a reduction in the development cycles for new products in the catalyst, polymer, electronic, optical material and semiconductor industries.

Customers also use our XRD systems in academic and government research, as well as in a variety of other fields, including forensics, art and archaeology.

XRF systems determine the elemental composition of a material and provide a full qualitative and quantitative analysis.

Our XRF systems direct X-rays at a sample, and the atoms in the sample absorb the X-ray energy.

Used in academic and government research, as well as in a variety of other fields, including forensics, art and archaeology.

XRF—X-ray fluorescence, also called X-ray spectrometry, including handheld XRF systems

Determine the elemental composition of a material and provide a full qualitative and quantitative analysis.

Direct X-rays at a sample, and the atoms in the sample absorb the X-ray energy.

The elements in the sample then emit X-rays that are characteristic for each element.

The system collects the X-rays, and the software analyzes the resulting data to determine the elements that are present.

Our XRF products provide automated solutions on a turn-key basis for industrial users that require

automated, controlled production processes that reduce product and process cost, increase output and improve product quality.

Our XRF products cover substantially all of the periodic table and can analyze solid, powder or liquid samples.

SC-XRD systems determine the three-dimensional structures of molecules in a chemical, mineral, or biological substance being analyzed.

Provide automated solutions on a turn-key basis for industrial users that require automated, controlled production processes that reduce product and process cost, increase output, and improve product quality.

Cover substantially all of the periodic table and can analyze solid, powder or liquid samples.

Used in academia, as well as in industry for research and quality and process control.

Industrial segments include pharma, metals, cement, petrochemistry, minerals, mining, and food, security, and environmental.

SC-XRD—Single crystal X-ray diffraction, often referred to as X-ray crystallography

Determine the three-dimensional structures of molecules in a chemical, mineral, or biological substance being analyzed.

SC-XRD systems have the capability to determine structure in both small chemical molecules and larger biomolecules.

SC-XRD systems direct an X-ray beam at a solid, single crystal sample.

Direct an X-ray beam at a solid, single crystal sample.

The atoms in the crystal sample scatter the X-rays to create a precise diffraction pattern recorded by an electronic detector.

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This information on the exact arrangement of atoms in the sample is a critical part of molecular analysis and can provide insight into a variety of areas, including how a protein functions or interacts with a second molecule.

Our SC-XRD systems are designed for use in the life sciences industry, academic research and a variety of other applications.

µCT is X-ray imaging in 3D, by the same method used in hospital CT scans, but on a small scale with massively increased resolution.

Used in the life sciences industry, academic research, and a variety of other applications.

µCT—X-ray micro computed tomography, X-ray microscopy

X-ray imaging in 3D, by the same method used in hospital CT scans, but on a small scale with massively increased resolution.

3D microscopy allows users to image the internal structure of objects non-destructively on a very fine scale.

Bruker µCT is available in a range of easy-to-use desktop instruments, which generate 3D images of the sample’s morphology and internal microstructure with resolution down to the sub-micron level.

Our µCT systems are used for numerous applications in materials research and in the life sciences industry.

EDS systems analyze the chemical composition of materials under investigation in electron microscopes by utilizing the fact that atoms of different chemical elements, when exposed to the high energy electron beam generated by the microscope, irradiate X-rays of different characteristic energy.

Used for numerous applications in materials research and in the life sciences industry.

EDS—Energy dispersive X-ray spectroscopy on electron microscopes

Used to analyze the chemical composition of materials under investigation in electron microscopes by utilizing the fact that atoms of different chemical elements, when exposed to the high energy electron beam generated by the microscope, irradiate X-rays of different characteristic energy.

The evaluation of the energy spectrum collected by our spectrometer allows the determination of the qualitative and quantitative chemical sample composition at the current beam position.

EDS systems allow for simultaneous analysis of all elements in the periodic table, beginning with atomic number 4 (beryllium).

Our EDS systems are used for a range of applications, including nanotechnology and advanced materials research, as well as materials analysis and quality control.

Customers for EDS systems include industrial customers, academia and government research facilities.

EBSD systems are used to perform quantitative microstructure analysis of crystalline samples in electron microscopes.

Used for a range of applications, including nanotechnology and advanced materials research, as well as materials analysis and quality control.

Customers include industrial customers, academia and government research facilities.

EBSD—Electron backscatter diffraction on electron microscopes

Used to perform quantitative microstructure analysis of crystalline samples in electron microscopes.

The microscope’s electron beam strikes the tilted sample and diffracted electrons form a pattern on a fluorescent screen.

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It provides the absolute crystal orientation with sub-micron resolution.

EBSD can be used to characterize materials with regard to crystal orientation, texture, stress, strain, and grain size.

EBSD also allows the identification of crystalline phases and their distribution and is applied to many industries such as metals processing, aerospace, automotive, microelectronics and earth sciences.

S-OES instruments are used for analyzing metals.

S-OES covers a broad range of applications for metals analysis from pure metals trace analysis to high alloyed grades and allows for analysis of a complete range of relevant elements simultaneously.

S-OES instruments pass an electric spark onto a sample, which burns the surface of the sample and causes atoms to jump to a higher orbit.

Used to characterize materials with regard to crystal orientation, texture, stress, strain, and grain size.

EBSD also allows the identification of crystalline phases and their distribution.

Used in many industries such as metals processing, aerospace, automotive, microelectronics, and earth sciences.

S-OES—Spark optical emission spectroscopy

Covers a broad range of applications for metals analysis from pure metals trace analysis to high alloyed grades and allows for analysis of a complete range of relevant elements simultaneously.

Instruments pass an electric spark onto a sample, which burns the surface of the sample and causes atoms to jump to a higher orbit.

Our detectors quantify the light emitted by these atoms and help our customers to determine the elemental composition of the material.

This technique is widely used in production control laboratories of foundries and steel mills.

CS/ONH carrier gas systems incorporate a furnace and infrared or thermal conductivity detection to analyze inorganic materials for the determination of carbon, sulfur, nitrogen, oxygen and hydrogen.

Combustion and inert gas fusion analyzers are used for applications in metal production and processing, chemicals, ceramics and cement, coal processing, oil refining and semiconductors.

STEM provides atomic-resolution information about physical and electronic structure, chemical identity, and local bonding environments by passing an atom-sized beam of electrons through a sample.

Used for analyzing metals in production control laboratories of foundries and steel mills.

CS/ONH—Combustion analysis for carbon, sulfur, oxygen, nitrogen, and hydrogen in solids

Carrier gas systems incorporate a furnace and infrared or thermal conductivity detection to analyze inorganic materials for the determination of carbon, sulfur, nitrogen, oxygen, and hydrogen.

Used for applications in metal production and processing, chemicals, ceramics and cement, coal processing, oil refining, and semiconductors.

STEM—Scanning transmission electron microscopes

Provides atomic-resolution information about physical and electronic structure, chemical identity, and local bonding environments by passing an atom-sized beam of electrons through a sample.

The beam is raster scanned, and a series of different detectors are used to make atomic-resolutions maps showing atomic locations, chemical species, shifts in valence states, and even vibrational modes (phonons).

Our STEM systems are used primarily in academic and national lab settings for basic science, advancing our fundamental understanding of the materials that will drive the next generation of technologies such as batteries, computer chips, and quantum information.

AFM systems provide atomic or near-atomic resolution of material surface topography using a nano-scale probe that is brought into light contact with the sample being investigated.

In addition to presenting a surface image, AFM can also provide quantitative nano-scale measurements of feature sizes, material properties, electrical information, chemical properties and other sample characteristics.

Our AFM systems are used for applications in academic and governmental materials and biological research and semiconductor, data storage hard drive, LED, battery, solar cells, polymers, and pharmaceutical product development and manufacturing.

FM products use fluorescence microscopy to determine the structure and composition of life science samples.

Used primarily in academic and national lab settings for basic science, advancing our fundamental understanding of the materials that will drive the next generation of technologies such as batteries, computer chips, and quantum information.

AFM—Atomic force microscopy

Provides atomic or near-atomic resolution of material surface topography using a nano-scale probe that is brought into light contact with the sample being investigated.

In addition to presenting a surface image, it can also provide quantitative nano-scale measurements of feature sizes, material properties, electrical information, chemical properties, and other sample characteristics.

Used for applications in academic and governmental materials and biological research and semiconductor, data storage hard drive, light emitting diode (“LED”), battery, solar cells, polymers, and pharmaceutical product development and manufacturing.

FM—Fluorescence microscopy

Our products include two-photon microscopes, multipoint scanning confocal microscopes, miniature head-mounted microscopes, super-resolution microscopes, light-sheet microscopes, laser illumination sources, photoactivation, photostimulation and photoablation accessories, and synchronization and analysis software.

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Multipoint scanning confocal systems allow live cell imaging with rapid acquisition of images for structural and composition analysis.

Miniature head-mount microscopes allow monitoring of animal brain activity during free-roaming, naturalistic

behavior at cellular level.

Miniature head-mount microscopes allow monitoring of animal brain activity during free-roaming, naturalistic behavior at cellular level.

Super-resolution and single-molecule localization microscopy products allow imaging below the optical diffraction limit by an order of magnitude.

Light-sheet based products allow fast 3D volume imaging with very low phototoxicity and photo-damage effects enabling live cell and large volume imaging.

SOM systems provide atomic or near-atomic two dimensional and three-dimensional surface resolution using white light interferometry, confocal optical and stylus profilometry methods.

SOM profilers range from low-cost manual tools for single measurements to advanced, highly automated systems for production line quality assurance and quality control applications where the combination of throughput, repeatability and reproducibility is essential.

SOM profilers support a range of applications in research, product development, tribology, quality control and failure analysis related to materials and machining in the automotive, orthopedic, ophthalmic, high brightness LED, semiconductor, data storage, optics and other markets.

TMT systems provide a platform for all types of common mechanical, friction, durability, scratch and indentation tests for a wide spectrum of materials.

Tribology systems are utilized for both academic research of the fundamental material properties and industrial applications in the semiconductor, aerospace, petroleum, automotive and other industries.

NanoIR systems perform infrared (IR) spectroscopy at the nanoscale.

Used to determine the structure and composition of life science samples.

SOM—Stylus and optical metrology

Provides atomic or near-atomic two dimensional and three-dimensional surface resolution using white light interferometry, confocal optical and stylus profilometry methods.

Range from low-cost manual tools for single measurements to advanced, highly automated systems for production line quality assurance and quality control applications where the combination of throughput, repeatability and reproducibility is essential.

Supports a range of applications in research, product development, tribology, quality control and failure analysis related to materials and machining in the automotive, orthopedic, ophthalmic, high brightness LED, semiconductor, data storage, optics, and other markets.

TMT—Tribology and mechanical test systems for analysis of friction and wear

Provides a platform for all types of common mechanical, friction, durability, scratch, and indentation tests for a wide spectrum of materials.

Utilized for both academic research of the fundamental material properties and industrial applications in the semiconductor, aerospace, petroleum, automotive, and other industries.

NanoIR—Nanoscale infrared spectroscopy

Performs infrared spectroscopy at the nanoscale.

Our systems use nanoprobe technology similar to what is used in our atomic force microscopes to deliver quantitative chemical information from the nanoscale to the sub-micron and macro scales.

The NanoIR measurement gives the user varying physical and chemical properties with nanoscale spatial resolution in a diverse range of fields, including polymers, 2D materials, materials science, life science and the micro-electronics industry.

The NanoIR measurement gives the user varying physical and chemical properties with nanoscale spatial resolution.

Our systems allow nanoscale IR absorption spectroscopy with interpretable IR spectra that directly correlates to FTIR as well as the complementary technique of nanoscale s-SNOM.

With our broadband sources, these systems allow broadband scientific spectroscopy.

Alicona systems combine the functionalities of a micro coordinate measurement machine (CMM) with those of a surface measurement system.

Used in a diverse range of fields, including polymers, 2D materials, materials science, life science, and the micro-electronics industry.

Alicona—Focus variation optical technology for non-contact dimensional metrology

Combines the functionalities of a micro coordinate measurement machine (“CMM”) with those of a surface measurement system.

These dimensional metrology systems are based on the pioneering development of optical focus-variation measurement algorithms and provide the noncontact measurement of form and roughness of complex, miniaturized geometries.

These systems serve many quality assurance application areas requiring precision measurement and dimensional metrology, including aerospace, automotive, precision medical products, additive manufacturing, and micro precision manufacturing.

Canopy provides spatial profiling services and instruments which include both our Cellscape instrument and ChipCytometry service for quantitative, high plex, targeted spatial proteomics in single cell and tissues.

These technologies, along with Canopy’s more basic IHC and FISH services, allow researchers to elucidate gene and protein expression in a spatial context, which is useful for deep biological insight into gene expression and for the development of biomarkers.

Canopy also provides transcriptional and multi-omic profiling services covering a variety of assays, including RNASeq and qPCR.

BCA provides customers with, among other offerings, Optofluidic platforms such as the Beacon and Beacon Select as well as Proteomic Barcoding platforms, such as the IsoLight System and the IsoSpark System.

Used in many quality assurance application areas requiring precision measurement and dimensional metrology, including aerospace, automotive, precision medical products, additive manufacturing, and micro precision manufacturing.

BCA—Optofluidic platforms and Proteomic Barcoding platforms

Provides customers with, among other offerings, Optofluidic platforms such as the Beacon and Beacon Discovery as well as Proteomic Barcoding platforms, such as the IsoLight System and the IsoSpark System.

These platforms provide scientists with opportunities to perform cell biology research through experiments using our proprietary consumables.

NanoString provides the CosMx Spatial Molecular Imager and GeoMx Digital Spatial Profiler for interrogating spatial transcriptomics;

and the nCounter Analysis System for quantitation of RNA-based gene expression.

These technologies allow researchers to measure gene expression in a spatial context at the regional and single-cell level, and to quantify RNA gene expression in a targeted manner, respectively.

Together, these tools are useful for characterizing the underlying biology of organs and tissues, the spatial gene expression associated with various disease states, and for deriving insights to drive the development of biomarkers.

Used for numerous applications in the life sciences industry and in academic research for assessing the functional phenotypes of single cells.

Canopy—Multiplexed fluorescence-based single cell imaging as well as multi-omics sample characterization

Provides spatial profiling services and instruments which include both our Cellscape instrument and ChipCytometry service for quantitative, high plex, targeted spatial proteomics in single cell and tissues.

These technologies, along with Canopy’s more basic IHC and FISH services, allow researchers to elucidate gene and protein expression in a spatial context.

Also provides transcriptional and multi-omic profiling services covering a variety of assays, including RNASeq and qPCR.

Useful for deep biological insight into gene expression and for the development of biomarkers.

NanoString—Platforms for spatial transcriptomic and multi-omic analysis and for the measurement of gene expression through detection of RNA and protein abundance

Provides the CosMx Spatial Molecular Imager and GeoMx Digital Spatial Profiler for interrogating spatial transcriptomics and multi-omics and the nCounter Analysis System for quantitation of RNA-based gene expression.

These technologies allow researchers to measure gene expression in a spatial context at the regional and single-cell level, and to quantify RNA gene expression in a targeted manner.

Useful for characterizing the underlying biology of organs and tissues, the spatial gene expression associated with various disease states, and for deriving insights to drive the development of biomarkers.

Bruker Spatial Genomics—Platform for single-cell, multiplexed visualization of the 3D architecture of the genome

Provides the PaintScape Spatial 3D Genome platform for the visualization of 3D genome and chromosomal structure at the single-cell level.

Allows researchers to gain novel insights into the underlying causes of disease stemming from chromosomal and chromatin-state dysregulation, mislocalization of genomic loci, and other spatial genomic features that can drive aberrant cellular function.

The BEST Segment designs, manufactures, and distributes superconducting materials, such as metallic low temperature superconductors (“LTS”) and high temperature superconductors (“HTS”), for use in magnetic resonance imaging, nuclear magnetic resonance, fusion energy research, and other applications in medical, clinical, pharmaceutical, high-energy physics, renewable energy, and environmental research.

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Sales and Marketing

We maintain direct sales forces throughout North America, Europe, China, Japan, and elsewhere in the Asia Pacific region.

We maintain direct sales forces throughout North America, Europe, China, Japan, and elsewhere in the Asia Pacific region, and in certain countries in Africa and South America.

We also utilize indirect sales channels to reach customers.

We have various international distributors and independent sales representatives in parts of Asia, Latin America, Africa, the Middle East and Eastern Europe.

We have various

international distributors and independent sales representatives in parts of Asia, Latin America, Africa, the Middle East, and Eastern Europe.

These entities augment our direct sales force and provide coverage in areas where we do not have direct sales personnel.

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In molecular diagnostics, CALID competes with a number of companies offering products for infectious disease diagnostics.

CALID also competes with a variety of companies that offer molecular spectrometry-based systems, including Thermo Fisher Scientific, PerkinElmer, Agilent, Foss, ABB Bomem, Buchi, Shimadzu, Horiba, Rigaku and Jasco.

CALID’s competitors in molecular spectrometry-based systems include Thermo Fisher Scientific, PerkinElmer, Agilent, Foss, ABB Bomem, Buchi, Shimadzu, Horiba, Rigaku, and Jasco.

CALID’s CBRNE detection customers are highly fragmented, and it competes with a number of companies in this area, of which the most significant competitor is Smiths Detection.

The BSI NANO Segment competes with companies that offer analytical X-ray solutions, OES systems, AFM and SOM systems and optical fluorescence systems, primarily Rigaku, Oxford Instruments, Agilent, Thermo Fisher Scientific, Ametek’s Spectro and Edax divisions, PANalytical, Park Systems, Olympus, Nikon, Zeiss and Danaher’s Leica business.

BEST competes with Western Superconducting Technologies Co., Ltd.

(WST), Luvata, and Jastec Co., Ltd.

in low temperature superconducting materials.

BEST further competes with Zanon, Mitsubishi Electric and AES in the development and supply of accelerator cavities, with Thales, Toshiba and CPI International in the development and supply of radio frequency couplers, with Mitsubishi Heavy Industries in the development and supply of superconducting accelerator modules and with AES and Thales for electron linear accelerators.

The BSI NANO Segment competes with companies that offer analytical X-ray solutions, OES systems, AFM and SOM systems, optical fluorescence systems, and genomics tools, primarily Rigaku, Oxford Instruments, Agilent, Thermo Fisher Scientific, Ametek’s Spectro and Edax divisions, PANalytical, Park Systems, Olympus, Nikon, Zeiss, 10x Genomics, Thorlabs, Bio-Techne, and Danaher’s Leica business.

BEST competes with Western Superconducting Technologies Co., Ltd., Luvata, and Jastec Co., Ltd.

in low temperature superconducting materials, with Zanon, Mitsubishi Electric, and AES in the development and supply of accelerator cavities, with Thales, Toshiba, and CPI International in the development and supply of radio frequency couplers, with Mitsubishi Heavy Industries in the development and supply of superconducting accelerator modules, and with AES and Thales for electron linear accelerators.

Manufacturing and Suppliers

Several of our manufacturing facilities are certified under ISO 9001:2015 international quality standards.

We manufacture and test our magnetic resonance products at our facilities in Faellanden, Switzerland;

Wissembourg, France;

and Karlsruhe, Germany.

We manufacture and test our preclinical imaging products at our facilities in Ettlingen, Germany;

Wissembourg, France;

Kontich, Belgium;

and Faellanden, Switzerland.

We manufacture and test our automation solutions at our facility in Basel, Switzerland.

We manufacture and test our mass spectrometry products at our facilities in Bremen, Germany.

We principally manufacture and test our molecular spectroscopy products, including CBRNE detection products, at our facilities in Ettlingen, Leipzig and Erlangen, Germany as well as Mississauga, Canada, Wallisellen, Switzerland and Osaka, Japan.

We manufacture and test our X-Ray products at our facilities in Karlsruhe, Berlin and Geesthacht, Germany;

Migdal HaEmek, Israel;

Kontich, Belgium;

Kirkland, Washington, USA, and Penang, Malaysia.

Our S-OES products are manufactured at our sites in Karlsruhe, Germany and Penang, Malaysia.

AFM instruments and components are manufactured at Santa Barbara and Camarillo, California, USA;

Berlin, Germany;

and Penang, Malaysia.

We manufacture and test the majority of our energy and superconducting products at our facilities in Hanau, Germany;

Bergisch Gladbach, Germany;

Perth, Scotland;

and Carteret, New Jersey, U.S.A.

Manufacturing processes at our facilities in Europe, Israel and California, U.S.A.

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We typically manufacture critical components in-house to ensure key competence and outsource to third party manufacturers non-critical components.

Refer to Item 2, Properties in this Annual Report on Form 10-K for further detail on our principal locations and products they manufacture.

We purchase materials and components from various suppliers that are either standard products or built to our specifications.

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Research and Development

We commit substantial capital and resources to internal and collaborative research and development projects in order to provide innovative products and solutions to our customers.

We conduct research primarily to enhance system performance and improve the reliability of existing products, and to develop revolutionary new products and solutions.

We are committed to ongoing innovation as a core element of our strategy to drive scientific research, develop new markets, enhance our product and service offerings, and to maintain our leadership position and relevance in the various industries in which we compete.

To support these efforts, we commit substantial resources to internal and collaborative research and development projects.

This level of investment in research and development has historically exceeded the industry average and reflects our commitment to scientific research and technological advancement to provide innovative products and solutions to our customers and maintain our competitive advantage.

We conduct research primarily to enhance system performance and improve the reliability of existing products, develop revolutionary new products and solutions, and to maintain technical competencies in our core technology platforms.

Our research and development efforts are conducted for the relevant products within each of the operating segments, as well as in collaboration with others on areas such as microfluidics, automation and workflow management software.

We have been the recipient of government grants from Germany and the United States for various projects related to early-stage research and development.

We have been the recipient of government grants within the European Union, Switzerland, and the United States for various projects related to research and development.

We have generally retained, at a minimum, non-exclusive rights to any items or enhancements we develop under these grants.

The German government requires that we use, and market technology developed under grants in order to retain our rights to the technology.

We have also accepted some sponsored

research contracts from private sources.

We have also accepted some sponsored research contracts from private sources.

Additionally, the Company has been able to gain access to research and development capabilities through acquisitions, acquiring the intellectual property, technology, and expertise of the acquired companies.

Refer to Item 2, Properties in this Annual Report on Form 10-K for further detail on our principal locations that are also used for research and development activities.

Our significant research and development activities (by segment) are as follows:

BSI BioSpin Segment:

The research and development performed in the BSI BioSpin Segment is primarily conducted at our facilities in Ettlingen, Germany;

Faellanden, Switzerland and Wissembourg, France.

The BSI BioSpin Segment maintains technical competencies in core magnetic resonance technologies and single- and multimodal imaging technologies and capabilities, including NMR, EPR, MRI, MPI, PET and CT.

The most recent technological innovations included Bruker’s ultra-high-field class (1GHz and above) in the NMR and MRI product lines and the benchtop Fourier NMR platform.

As part of our continuous development of systems and software, we have achieved major milestones in 2023 to make NMR and MRI technologies accessible to more labs, such as reduced siting requirements.

BSI CALID Segment:

The research and development performed in the BSI CALID Segment is primarily conducted at our facilities in Bremen, Ettlingen, Leipzig and Erlangen, Germany;

Faellanden and Wallisellen, Switzerland;

Toronto, Canada;

Osaka, Japan, Wissembourg, France;

Athens, Greece;

and Torino, Italy.

The BSI CALID Segment maintains technical competencies in core mass spectrometry technologies and capabilities, including:

MALDI, ESI and EI/CI ion source, TOF, TOF/TOF, ion traps, MRMS, quadrupole and IMS analyzers and bioinformatics.

Recent projects include the innovative timsTOF mass spectrometer for separation and analysis of unresolved compounds and conformations and further enhanced instrument sensitivity.

The BSI BioSpin Segment’s most recent technological innovations included Bruker’s ultra-high-field class (1GHz and above) in the NMR and MRI product lines and the benchtop Fourier NMR platform.

As part of our continuous development of systems and software, we have achieved many major milestones to make NMR and MRI technologies accessible to more labs, such as reduced sitting requirements.

The BSI CALID Segment’s recent projects include the innovative timsTOF mass spectrometer for separation and analysis of unresolved compounds and conformations, and further enhanced instrument sensitivity.

In addition, new sample preparation and automation workflows were developed for deep plasma proteomics (Biognosys P2 workflow and Preomics ENRICHplus kit).

The BSI CALID Segment also maintains technical competencies in core vibrational spectroscopy technologies and capabilities, including FT-IR, NIR and Raman.

BSI NANO Segment:

The research and development performed in the BSI NANO Segment is primarily conducted at our facilities in Karlsruhe, Berlin and Leipzig, Germany;

Penang, Malaysia;

Madison, Wisconsin, Eden Prairie, Minnesota, San Jose, Santa Barbara and Emeryville, California, Bothell, Washington, and St.

Louis, Missouri, U.S.A.

The BSI NANO Segment maintains technical competencies in core X-ray technologies and capabilities, including detectors used to sense X-ray and X-ray diffraction patterns, X-ray sources and optics that generate and focus the X-rays, robotics and sample handling equipment that holds and manipulates the experimental material, and software that generates the structural data.

Recent projects include fluorescence microscopy with simultaneous, all-optical stimulation and imaging platforms for optogenetics neuroscience research and light sheet cell microscopy systems, which enable brain research and high-resolution live cell research and single-cell biology research tools to deliver deep insights into cellular function and new perspectives on phenomes and genotype-to-phenotype linkages.

The BSI NANO Segment also has competencies in AFM technology, which involve sub-angstrom level position and motion control, as well as sub-pico newton force control.

The BSI NANO Segment technologies also include 3D optical inference-based microscopy, stylus profilometry, tribology testing, nano-indentation, optical fluorescence two-photon microscopy, multipoint scanning microscopy, high-speed, 3D super-resolution florescence microscopy and spatial biology and single-cell targeted proteomics technologies.

Recent innovations include elemental analyzer systems for advanced applications and research and simultaneous, all-optical stimulation and imaging platforms for neuroscience applications.

BEST Segment:

The research and development performed in the BEST Segment is primarily conducted at our facilities in Hanau and Bergisch Gladbach, Germany;

and Carteret, New Jersey, U.S.A.

BEST maintains technical competencies in the production and development of low and high temperature superconducting materials and devices.

The BSI NANO Segment’s recent innovations include an EBSD detector based on direct electron detection for ultra-fast and ultra-sensitive characterization of crystalline materials in electron microscopes, transmission electron microscopes for investigations under non-ambient conditions, an X-ray µCT system that delivers functionality of a floor standing system in a compact bench-top design, high-performance fully automated uXRD and uXRF X-ray systems for AI semiconductor chip yield management and automated AFM metrology systems used in the production of High Bandwidth Memory (“HBM”) for AI applications, advancements to imaging-based or digital readout platforms for spatial biology applications, including spatial proteomics and spatial transcriptomics, spatial 3D genome and multiomic capabilities, single-cell live functional analysis, high-speed neural imaging, deep organoid imaging, and expanded field of view for connectivity studies in multiphoton microscopy.

BEST has had unique innovations in the production and development of low and high temperature superconducting materials and devices.

Intellectual Property

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As a result, we cannot predict the enforceability of our patents with certainty.

In addition, we are

aware of the existence from time to time of patents in certain countries, which, if valid, could impair our ability to manufacture and sell products in these countries.

In addition, we are aware of the existence from time to time of patents in certain countries, which, if valid, could impair our ability to manufacture and sell products in these countries.

We also rely upon trade secrets, know-how, trademarks, copyright protection, and licensing to develop and maintain our competitive position.

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Government Regulation

We are required to comply with federal, state, and local environmental protection regulations.

We are required to comply with international and U.S.

federal, state, and local environmental protection regulations.

We do not expect this compliance to have a significant impact on our capital spending, earnings or competitive position.

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In some countries where Bruker AXS sells systems, Bruker AXS uses the certificate that Bruker AXS obtained from the federal authorities in Germany to assist it in obtaining a license, registration or certificate, as applicable, from the country or its relevant authorities in which the sale occurs.

Our Bruker AXS subsidiary possesses low-level radiation materials licenses from the local radiation safety authority, Gewerbeaufsichtsamt Karlsruhe, for its facility in Karlsruhe, Germany, and from the local radiation safety authority, Kanagawa Prefecture, for its facility in Yokohama, Japan, as well as from various other countries in which it sells its products.

Our Bruker Optics subsidiary possesses low-level radiation licenses for facilities in Billerica, Massachusetts and Ettlingen and Leipzig, Germany.

Nuclear Regulatory Commission also has regulations concerning the exposure of our employees to radiation.

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Should we pursue an FDA or comparable foreign regulatory authority clearance, authorization, or approval for a new device or device modification, we cannot be certain that we will receive required clearance, authorization, or approval on a timely basis or at all.

The failure to receive clearance, authorization, or approval for

significant new products or modifications to existing products on a timely basis or at all could have a material, adverse effect on our financial condition and results of operations.

The failure to receive clearance, authorization, or approval for significant new products or modifications to existing products on a timely basis or at all could have a material, adverse effect on our financial condition and results of operations.

Both before and after a medical device product is commercially released, we have ongoing responsibilities under FDA and foreign regulations.

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The regime changes significantly with the IVDR.

In comparison to the IVDD, the IVDR requires, among other things, more clinical evidence to demonstrate the claimed benefits and safety of the device in relation to its stated purpose, stricter classification and CE-marking requirements and ongoing post-market follow-up to ensure conformity.

follow-up to ensure conformity.

The IVDR also requires new databases to be set up to track which devices are CE marked and to register clinical studies and post-market monitoring.

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Our products that are currently approved under the IVDD and not already placed on the market or put into service, must be recertified under the IVDR.

Our backlog consists of firm orders under non-cancelable purchase orders received from customers.

Total remaining performance obligations as of December 31, 2024, and 2023 was approximately $2,090.4 million and $2,226.7 million, respectively.

The decrease in our backlog in 2024 compared to 2023 was primarily driven by lower BEST order bookings.

Our backlog consists of firm orders (also referred to as bookings) under non-cancelable purchase orders received from customers for instruments and services.

Total remaining performance obligations as of December 31, 2025, and 2024 were approximately $2,569.4 million and $2,090.4 million, respectively.

The increase in our backlog in 2025 compared to 2024 was primarily driven by higher BEST order bookings.

We generally experience varying revenue levels in the first three quarters of the year, while our fourth quarter revenues have historically been stronger than the rest of the year.

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Our employees are a critical component of that mission.

We endeavor to attract, develop and retain top talent by offering our employees a challenging but rewarding work experience, as well as competitive compensation and benefits.

We endeavor to attract, hire, and retain top talent by offering our employees a challenging but rewarding work experience, as well as competitive compensation and benefits.

Further, we strive to create a work environment that promotes integrity, respect, and trust among our employees.

Workforce Composition

As of December 31, 2024, and 2023, Bruker employed the following full-time employees:

Number of Employees

Full-time employees in the United States

Full-time employees in other countries

Total employees

The table below provides a breakdown of Bruker’s employees by functional area.

Number of Employees

Production and distribution employees

Selling and marketing employees

General and administrative employees

Research and development employees

Total employees

As of December 31, 2025, Bruker had approximately 11,085 employees worldwide.

Approximately 20.2% of our employees were located in the United States.

Additionally, approximately 45.8% of our employees were in production and distribution, 23.5% in selling and marketing, 17.6% in research and development and 13.1% in general and administrative functions.

Talent, Development and Workforce Strategy

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These employees are encouraged to continue their professional development with additional coaching and on the job learning opportunities to enhance their readiness for future advancement.

In 2024, we continued with our supervisor training program for newly hired or promoted first line leadership.

This program is expanding to other employee groups within Bruker to provide a consistent foundation of knowledge and expectations for our leaders.

Additionally, our robust leadership program brings together participants from across the globe to provide education aimed to enhance their skills in business acumen, leadership, and communication.

A practical opportunity to apply these skills is included as part of the program under the mentorship of experienced leaders.

The coaching from internal leaders is supported also by professional external mentorship ensuring a comprehensive learning experience.

In 2025, we continued with several supervisor and leadership training programs for eligible participants.

These programs provide a consistent foundation of knowledge and expectations for our leaders across Bruker.

Employee Engagement

Bruker is committed to fostering a culture of continuous learning and knowledge sharing to support development and retention, and also recognizes the importance of inclusion.

Inclusion is an essential element of our core values and is crucial to the dynamic, entrepreneurial working environment our employees thrive in, and aligns with our mission to innovate responsibly and with integrity.

We support several initiatives that promote inclusion at Bruker:

Bruker is committed to fostering a culture of continuous learning and knowledge sharing to support development and retention and also recognizes the importance of a culture of belonging.

A strong sense of belonging is crucial to the dynamic, entrepreneurial working environment our employees thrive in, and aligns with our mission to innovate responsibly and with integrity.

We support several initiatives that promote belonging at Bruker:

• Cross-functional collaboration and teamwork, encouraging employees to build relationships across different business units and geographies.

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Our employees play active roles in these initiatives, often creating and facilitating them directly.

Bruker has continued to support events such as Germany’s nationwide Girls’ Day and celebration of the festival of colors, HOLI, in India.

These programs and events help employees develop their skills, expand their networks, and contribute meaningfully to our business objectives.

They also provide critical connections for new hires and establish internal networks across functions for employees who may not otherwise have opportunity to interact.

We encourage these activities to help employees develop their skills, expand their networks, and contribute meaningfully to our business objectives.

provide critical connections for new hires and establish internal networks across functions for employees who may not otherwise have opportunity to interact.

Employee Health and Safety

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Bruker accomplishes this through compliance with applicable laws and regulations regarding workplace safety, including recognition and control of workplace hazards, the elimination of unsafe manufacturing and workplace practices, tracking injury and illness rates, utilizing a global travel health program, and maintaining detailed emergency and disaster recovery plans.

We are committed to reducing safety risks across our business groups and at our

corporate and manufacturing sites worldwide.

We are committed to reducing safety risks across our business groups and at our corporate and manufacturing sites worldwide.

The following are examples of initiatives and programs designed with employee health and safety in mind:

• One of our German facilities has a robust accident prevention program and contributes to active environmental protection.

This has been recognized with an ISO 45001 certification.

• A confidential Employee Assistance Program has been implemented at several new sites providing no-cost support to employees and offering assistance on several personal topic areas.

• Many locations throughout the globe encourage employees to be mindful of a healthy lifestyle such as organized group exercise activities.

These activities not only enhance employee physical health, but emotional and mental health as well through increasing camaraderie among colleagues.

• Many locations throughout the globe encourage employees to be mindful of a healthy lifestyle, including by offering group exercise activities.

These activities aim to enhance employee physical health, emotional and mental, as well to increase camaraderie among colleagues.

Available Information

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Compared sentence by sentence after normalising whitespace, quotation marks, case and digits, so re-formatting and restated figures do not read as changed language. Wording changes appear as one removal and one addition. The current filing and the prior one are authoritative.

What changed 10-K

Item 1. Business